Fipronil induces apoptosis and cell cycle arrest in porcine oocytes during in vitro maturation

• Published in: Apoptosis (London) 2019-10, Vol.24 (9-10), p.718-729
• Main Authors: Zhou, Wenjun, Niu, Ying-Jie, Nie, Zheng-Wen, Kim, Yong-Han, Shin, Kyung-Tae, Guo, Jing, Cui, Xiang-Shun
• Format: Article
• Language: English
• Subjects: Apoptosis; Bcl-x protein; Biochemistry; Biomedical and Life Sciences; Biomedicine; Cancer Research; Cell Biology; Cell cycle; Chloride channels; Chloride ions; Cyclin B1; Cytochrome c; Cytochromes; Deoxyribonucleic acid; DNA; DNA damage; Extrusion rate; Females; Fipronil; GABA; Gametes; Gametocytes; Insecticides; Ion channels; Mammals; Maturation; Membrane potential; Mitochondria; Oncology; Oocytes; p53 Protein; Pesticides; Pests; Phenylpyrazole; Reactive oxygen species; Reproductive system; Tumor proteins; Virology; γ-Aminobutyric acid
• ISSN: 1360-8185; 1573-675X
• DOI: 10.1007/s10495-019-01552-w

Fipronil (FPN) is a widely used phenylpyrazole pesticide that can kill pests by blocking γ-aminobutyric acid (GABA)-gated chloride channels. In addition, there are lack of studies on the effects of FPN on the female mammalian gametes. In this study, porcine oocytes were used to investigate the effects of FPN on the oocyte maturation process. The results showed that the first polar body extrusion rate significantly decreased (100 μM FPN vs. control, 18.64 ± 2.95% vs. 74.90 ± 1.50%, respectively), and oocytes were arrested at the germinal vesicle stage in 100 μM FPN group. Meanwhile, the FPN caused a significant increase in reactive oxygen species (ROS) levels and severe DNA damage inside the oocytes. Furthermore, apoptosis was enhanced along with decreases in mitochondrial membrane potential, BCL-xL, and the release of cytochrome C in FPN-treated group. Additionally, low CDK1 activity and delayed cyclin B1 degradation during germinal vesicle breakdown were found in the FPN-treated group, which resulted from the activation of ATM-P53-P21 pathway. In conclusion, FPN induces apoptosis and cell cycle arrest in porcine oocyte maturation because of increased ROS levels and DNA damage. This suggests that the FPN in the environment may have potential detrimental effects on the female mammalian reproductive system.