Effects of phenanthrene on oxidative stress and inflammation in lung and liver of female rats

• Published in: Environmental toxicology 2020-01, Vol.35 (1), p.37-46
• Main Authors: Ma, Haitao, Wang, Huiling, Zhang, Haojun, Guo, Huizhen, Zhang, Wenwen, Hu, Fengjing, Yao, Yueli, Wang, Dong, Li, Chengyun, Wang, Junling
• Format: Article
• Language: English
• Subjects: 8-Hydroxydeoxyguanosine; Air Pollutants - toxicity; Animals; Corn oil; Cytokines; Cytokines - blood; Cytokines - immunology; Deoxyguanosine; Dosage; Dose-Response Relationship, Drug; Female; Females; Forkhead protein; Foxp3 protein; Gene expression; Growth factors; Inflammation; inflammatory cytokines; Injury analysis; Interleukin 10; Interleukin 6; Interleukins; Liver; Liver - drug effects; Liver - immunology; Liver - metabolism; lung; Lung - drug effects; Lung - immunology; Lung - metabolism; Lungs; Malondialdehyde; Necrosis; Neoplasms; Oils & fats; Oxidative stress; Oxidative Stress - drug effects; Oxidative Stress - immunology; Phenanthrene; Phenanthrenes - toxicity; Pilot Projects; Proteins; Random Allocation; Rats; Rats, Wistar; Rodents; Serum; Superoxide dismutase; Transcription; Transforming growth factor-b; Tumor necrosis factor; Tumor necrosis factor-TNF
• ISSN: 1520-4081; 1522-7278; 1522-7278
• DOI: 10.1002/tox.22840

Phenanthrene (Phe) female rat model was established to explore the effects of Phe on oxidative stress and inflammation. The rats were randomly divided into three groups including control (C), low (L), and high (H) group. Phe was supplied to L and H groups at the dosage of 180 mg/kg and 900 mg/kg orally at first day, and with the dose 90 mg/kg and 450 mg/kg by intraperitoneal injection at the last 2 days. The C group was enriched with the same volume of corn oil. The blood, lung, and liver tissues were collected. The superoxide dismutase (SOD), malonaldehyde (MDA), and 8‐hydroxy‐2‐deoxyguanosine (8‐OHdG) were detected to evaluate oxidative stress. The protein and mRNA expressions of interleukin 6 (IL‐6), tumor necrosis factor‐α (TNF‐α), transforming growth factor‐β (TGF‐β), and interleukin 10 (IL‐10) were detected to evaluate inflammation. Further, the forkhead box transcription factor 3 (Foxp3) was analyzed to hint the injury mechanism of inflammation. The results showed SOD and MDA in lung and liver, and serum 8‐OHdG elevated significantly in H groups (P