Multifunctional Regulation of 3D Cell-Laden Microsphere Culture on an Integrated Microfluidic Device

Three-dimensional (3D) hydrogel microspheres have aroused increasing attention as an in vitro cell culture model. Yet the preservation of cells’ original biological properties has been overlooked during model construction. Here we present an integrated microfluidic device to accomplish the overall p...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2019-10, Vol.91 (19), p.12283-12289
Main Authors: Zheng, Yajing, Wu, Zengnan, Khan, Mashooq, Mao, Sifeng, Manibalan, Kesavan, Li, Nan, Lin, Jin-Ming, Lin, Ling
Format: Article
Language:English
Subjects:
Analytical chemistry
Angiogenesis
Biocompatibility
Biological properties
Cell culture
Chemistry
Damage accumulation
Hydrogels
Microfluidic devices
Microfluidics
Microspheres
Mitochondria
Oxidative stress
Preservation
Tissue engineering
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Summary:Three-dimensional (3D) hydrogel microspheres have aroused increasing attention as an in vitro cell culture model. Yet the preservation of cells’ original biological properties has been overlooked during model construction. Here we present an integrated microfluidic device to accomplish the overall process including cell-laden microsphere generation, online extraction, and dynamic-culture. The method extends the noninvasive and nonsuppression capabilities of the droplet preparation system and provides a constant microenvironment, which reduces intracellular oxidative stress damage and the accumulation of mitochondria. Compared to the conventional preparation method, the coculture model of tumor-endothelial construction on an integrated platform displays high-level angiogenic protein expression. We believe that this versatile and biocompatible platform will provide a more reliable analysis tool for tissue engineering and cancer therapy.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.9b02434