Magnetic Control of Axon Navigation in Reprogrammed Neurons

While neural cell transplantation represents a promising therapy for neurodegenerative diseases, the formation of functional networks of transplanted cells with host neurons constitutes one of the challenging steps. Here, we introduce a magnetic guidance methodology that controls neurite growth sign...

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Bibliographic Details
Published in:Nano letters 2019-09, Vol.19 (9), p.6517-6523
Main Authors: Jin, Yoonhee, Lee, Jung-uk, Chung, Eunna, Yang, Kisuk, Kim, Jin, Kim, Ji-wook, Lee, Jong Seung, Cho, Ann-Na, Oh, Taekyu, Lee, Jae-Hyun, Cho, Seung-Woo, Cheon, Jinwoo
Format: Article
Language:English
Subjects:
Antibodies - chemistry
Axons - metabolism
Cellular Reprogramming
DCC Receptor - antagonists & inhibitors
DCC Receptor - metabolism
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Magnetic Fields
Magnetite Nanoparticles - chemistry
Neurites - metabolism
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Summary:While neural cell transplantation represents a promising therapy for neurodegenerative diseases, the formation of functional networks of transplanted cells with host neurons constitutes one of the challenging steps. Here, we introduce a magnetic guidance methodology that controls neurite growth signaling via magnetic nanoparticles (MNPs) conjugated with antibodies targeting the deleted in colorectal cancer (DCC) receptor (DCC-MNPs). Activation of the DCC receptors by clusterization and subsequent axonal growth of the induced neuronal (iN) cells was performed in a spatially controlled manner. In addition to the directionality of the magnetically controlled axon projection, axonal growth of the iN cells assisted the formation of functional connections with pre-existing primary neurons. Our results suggest magnetic guidance as a strategy for improving neuronal connectivity by spatially guiding the axonal projections of transplanted neural cells for synaptic interactions with the host tissue.
ISSN:1530-6984
1530-6992
DOI:10.1021/acs.nanolett.9b02756