Endophytic bacteria of desert cactus (Euphorbia trigonas Mill) confer drought tolerance and induce growth promotion in tomato (Solanum lycopersicum L.)

• Published in: Microbiological research 2019-11, Vol.228, p.126302-126302, Article 126302
• Main Authors: Eke, Pierre, Kumar, Aundy, Sahu, Kuleshwar Prasad, Wakam, Louise Nana, Sheoran, Neelam, Ashajyothi, Mushineni, Patel, Asharani, Fekam, Fabrice Boyom
• Format: Article
• Language: English
• Subjects: Acclimatization; Bacillus - classification; Bacillus - genetics; Bacillus - isolation & purification; Bacillus - physiology; Bacterial endophytes; Biomass; Cactaceae - microbiology; Cactus; Cameroon; Desert Climate; DNA, Ribosomal - genetics; Drought tolerance; Droughts; Endophytes - classification; Endophytes - genetics; Endophytes - isolation & purification; Endophytes - physiology; Growth promotion; Lycopersicon esculentum - growth & development; Lycopersicon esculentum - microbiology; Phylogeny; Plant Development; Plant Leaves; Plant Roots; Rifamycins - pharmacology; RNA, Ribosomal, 16S - genetics; Sequence Analysis; Soil Microbiology; Stress, Physiological; Tomato
• ISSN: 0944-5013; 1618-0623
• DOI: 10.1016/j.micres.2019.126302

Endophytic bacteria isolated from cactus were characterized and assessed for their capability to induce drought tolerance and growth promotion in tomato. A total of 191-bacteria representing 13-genera and 18-species were isolated from wild cactus, Euphorbia trigonas. Bacillus (58), Lysinibacillus (36), Enterobacter (29), Stenotrophomonas (18), Lelliottia (12) and Pseudomonas (12) were the most represented genera. 16S rDNA sequence (>1400-bp) comparison placed the bacterial isolates with Bacillus xiamenensis; Bacillus megaterium; Bacillus cereus; Bacillus amyloliquefaciens; Bacillus velezensis; Brevibacillus brevis; Lysinibacillus fusiformis; Enterobacter cloacae; Lelliottia nimipressuralis; Proteus penneri; Sphingobacterium multivorum; Klebsiella pneumoniae; Pseudomonas putida; Pseudomonas aeruginosa; Stenotrophomonas maltophilia; Citrobacter freundii; Chryseobacterium indologenes and Paracoccus sp. Bacillus xiamenensis was identified for the first time as plant endophyte. Upon bacterization, the endophytes triggered germination and growth promotion in tomato as indicated by 118 % and 52 % more root-biomass under drought-free and drought-induced conditions, respectively. Bacillus amyloliquefaciens CBa_RA37 and B. megaterium RR10 displayed broad spectrum endophytism in tomato. Bacterization of tomato with cactus endophyte showed altered oxidative status, stomatal and photosystem II functioning, internal leaf temperature and relative water content suggestive of physiological de-stressing from moisture stress. Activity of oxidative stress enzymes such as guaiacol peroxidase and catalase was also indicative of endophyte assisted de-stressing of tomato. Re-irrigation on 20-days of drought infliction showed 86.9% recovery of B. amyloliquefaciens CBa_RA37 primed tomato when non-primed plantlets succumbed. The cactus endophytic bacterial strain B. amyloliquefaciens CBa_RA37 showed promise for low-cost, efficient and environmentally friendly bio-inoculant technology to mitigate drought in arid zones of Asian and African continents.