Effects of daphnetin combined with insulin-like growth factor 1 on chondrogenic differentiation of adipose-derived mesenchymal stem cells in rats

To investigate the effect of daphnetin (DAP) combined with insulin-like growth factor 1 (IGF-1) gene transfection on chondrogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs) in rats. Rat ADSCs were isolated and amplified by enzymatic digestion. The third generation ADSCs were t...

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Published in:Zhongguo xiu fu chong jian wai ke za zhi 2019-06, Vol.33 (6), p.743-749
Main Authors: Shen, Ning, Xi, Xiaoyun, Li, Fei, Lü, Chaoliang, Zhang, Pusheng
Format: Article
Language:Chinese
Subjects:
Adipose Tissue - cytology
Animals
Cell Differentiation
Cells, Cultured
Insulin-Like Growth Factor I - pharmacology
Mesenchymal Stem Cells - physiology
Rats
Transfection
Umbelliferones - pharmacology
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Summary:To investigate the effect of daphnetin (DAP) combined with insulin-like growth factor 1 (IGF-1) gene transfection on chondrogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs) in rats. Rat ADSCs were isolated and amplified by enzymatic digestion. The third generation ADSCs were treated with IGF-1 gene transfection as experimental group and normal ADSCs as control group. The cells of the two groups were treated with different concentrations of DAP (0, 30, 60, 90 μg/mL), respectively. Cell proliferation was detected by cell counting kit 8 (CCK-8) after cultured for 72 hours. After 14 days, real-time fluorescence quantitative PCR and Western blot were used to detect the mRNA and protein expressions of chondrocyte markers (collagen type Ⅱ and Aggrecan) in each group; and toluidine blue staining and collagen type Ⅱ immunohistochemical staining were performed. CCK-8 assay showed that with the increase of DAP concentration, the cell absorbance ( ) value of the control group and the experimental g
ISSN:1002-1892
DOI:10.7507/1002-1892.201812063