A Molecular Cytogenetic Map of Scallop (Patinopecten yessoensis)

To consolidate the genetic, physical, and cytogenetic maps of scallop ( Patinopecten yessoensis ), we constructed a molecular cytogenetic map by localizing 84 fosmid clones that contain different SNP markers from 19 linkage groups (LGs) using fluorescence in situ hybridization (FISH). Among these 84...

Full description

Saved in:
Bibliographic Details
Published in:Marine biotechnology (New York, N.Y.) N.Y.), 2019-12, Vol.21 (6), p.731-742
Main Authors: Yang, Zujing, Li, Xuan, Liao, Huan, Hu, Liping, Peng, Cheng, Wang, Shenhai, Huang, Xiaoting, Bao, Zhenmin
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Breeding
Chromosome Mapping
Chromosomes
Clones
Cloning
Colour
Comparative analysis
Comparative studies
Cytogenetics
Engineering
Fluorescence
Fluorescence in situ hybridization
Freshwater & Marine Ecology
Gene mapping
Genetic Linkage
Hybridization
In Situ Hybridization, Fluorescence
Life Sciences
Marine molluscs
Microbiology
Mollusks
Orientation
Original Article
Patinopecten yessoensis
Pectinidae - genetics
Polymorphism, Single Nucleotide
Single-nucleotide polymorphism
Zoology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To consolidate the genetic, physical, and cytogenetic maps of scallop ( Patinopecten yessoensis ), we constructed a molecular cytogenetic map by localizing 84 fosmid clones that contain different SNP markers from 19 linkage groups (LGs) using fluorescence in situ hybridization (FISH). Among these 84 SNP-anchored clones, 56 clones produced specific and stable signals on one pair of chromosomes. Dual-color FISH assigned 19 LGs to their corresponding chromosomes with 38 SNP-anchored clones as probes. Among these 19 LGs, 17 LGs were assigned to their corresponding one pair of chromosomes, while two clones containing SNPs from LG10 and LG19 were located on two different pairs of chromosomes separately. The orientation of 7 LGs was corrected according to the chromosome location of SNPs within the same LG. In addition, a probe panel of SNP-anchored clones was developed to identify each chromosome of P. yessoensis . The molecular cytogenetic map will facilitate molecular breeding in scallop and enable comparative studies on chromosome evolution of bivalve mollusk.
ISSN:1436-2228
1436-2236
DOI:10.1007/s10126-019-09918-6