Proteomic profiling of root system development proteins in chrysanthemum overexpressing the CmTCP20 gene

•Overexpression of CmTCP20 promotes both adventitious and lateral roots development in chrysanthemum.•CmTCP20 improves IAA content in chrysanthemum roots.•The proteins related to auxin, calcium signals, and cell wall were differentially expressed in the CmTCP20-overexpressing lines. Plant root syste...

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Bibliographic Details
Published in:Plant science (Limerick) 2019-10, Vol.287, p.110175-110175, Article 110175
Main Authors: Fan, Hong-Mei, Liu, Bo-Wen, Ma, Fang-Fang, Sun, Xia, Zheng, Cheng-Shu
Format: Article
Language:English
Subjects:
Auxin
Chlorophyll - metabolism
Chromatography, High Pressure Liquid
Chrysanthemum
Chrysanthemum - genetics
Chrysanthemum - growth & development
Chrysanthemum - metabolism
CmTCP20
Gas Chromatography-Mass Spectrometry
Gene Expression Regulation, Plant
Genes, Plant - physiology
Indoleacetic Acids - metabolism
Nitrate
Photosynthesis
Plant Growth Regulators - metabolism
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Roots - growth & development
Plant Roots - metabolism
Proteomics
Real-Time Polymerase Chain Reaction
Root system development
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:•Overexpression of CmTCP20 promotes both adventitious and lateral roots development in chrysanthemum.•CmTCP20 improves IAA content in chrysanthemum roots.•The proteins related to auxin, calcium signals, and cell wall were differentially expressed in the CmTCP20-overexpressing lines. Plant root systems ensure the efficient absorption of water and nutrients and provide anchoring into the soil. Although root systems are a highly plastic set of traits that vary both between and among species, the basic root system morphology is controlled by inherent genetic factors. TCP20 has been identified as a key regulator of root development in plants, and yet its underlying mechanism has not been fully elucidated, especially in chrysanthemum. We found that overexpression of the CmTCP20 gene promoted both adventitious and lateral root development in chrysanthemum. To get further insight into the molecular mechanisms controlling root system development, we conducted a study employing tandem mass tag proteomic to characterize the differential root system development proteomes from CmTCP20-overexpressing and wild-type chrysanthemum root samples. Of the proteins identified, 234 proteins were found to be differentially abundant (>1.5-fold cut off, p 
ISSN:0168-9452
1873-2259
DOI:10.1016/j.plantsci.2019.110175