Characterization of a novel Clostridium sp. SP17–B1 and its application for succinic acid production from hevea wood waste hydrolysate

• Published in: Anaerobe 2020-02, Vol.61, p.102096-102096, Article 102096
• Main Authors: Phuengjayaem, Sukanya, Tanasupawat, Somboon, Teeradakorn, Siriluk
• Format: Article
• Language: English
• Subjects: Clostridium; Detoxification; Granular activated carbon; Hevea wood waste hydrolysate; Succinic acid
• ISSN: 1075-9964; 1095-8274
• DOI: 10.1016/j.anaerobe.2019.102096

An anaerobic, gram-positive, rod-shaped bacterium strain SP17–B1, isolated from dog saliva, was taxonomically characterized on the basis of phenotypic, chemotaxonomic, and genotypic characteristics. It was cultured in 4% (w/v) NaCl at a pH range of 5.0–8.0 (optimally at pH 7) and at 30°C–40 °C (optimally at 37 °C). Its major cellular fatty acids are C16:0 (36.3%), C17:0 cyclo (9.7%), C16:1ω9c (13.9%), and C18:1ω9c (10.7%), and its DNA guanine–cytosine content is 40.8 mol%. On the basis of the 16S rRNA gene sequence analysis, it was determined that the strain belonged to the genus Clostridium and was closely related to C. amygdalinum BR-10T (97.8%), C. saccharolyticum WM1T (97.8%), and C. celleracrescens DSM 5628T (97.7%). This strain showed a low level of DNA–DNA relatedness with the closely related strains, suggesting that it is a novel species in the genus Clostridium. Recent studies have demonstrated the production of succinic acid using Clostridium strains. Strain SP17–B1 produced 25.1 ± 1.3 and 15.3 ± 1.5 g/L of succinic acid from 40 g/L of glucose and 30 g/L of hevea wood waste hydrolysate (HH), respectively, after 24 h. When detoxified HH was used as a substrate, the lag phase was reduced and cell growth was enhanced by 7 fold (OD660 0.4–3.0) within 12 h. Detoxification using granular activated carbon may have reduced the levels of furfural and HMF without interfering with the amount of sugars in HH. •This study characterized a new anaerobic bacterium, strain SP17-B1, isolated from dog saliva, based on phenotypic, chemotaxonomic and genotypic characteristics. Recent research has demonstrated the production of succinic acid by Clostridium strains.•Strain SP17-B1 produced 25.1 g/L and 15.3 g/L of succinic acid from 40 g/L of glucose and 30 g/L of HH, respectively.•Using detoxified HH as a substrate, the lag phase was reduced, and cell growth was enhanced 7-fold. Our study makes a significant contribution to the literature because thus far, the research in the production of succinic acid by Clostridium has been neglected.•The focus of our study is in line with your journal's scope; specifically, we describe an obligately anaerobic bacterium originally isolated from dog saliva and with novel capabilities.