Paternal bias expression of Igf2as is enhancer-dependent on the imprinting cluster of Igf2, H19 and Nctc1 in muscle cells

Igf2, H19, and Nctc1 are linked co-regulated genes on distal mouse chromosome 7. This locus is an important model both for studying mechanisms of monoallelic expression and for elucidating the role of cis-regulatory elements - enhancers and insulators - in organizing chromatin and gene expression ac...

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Bibliographic Details
Published in:Animal cells and systems 2019, 23(4), , pp.288-293
Main Authors: Kim, Jae-Young, Youn, Hwa Young, Pfeifer, Karl, Eun, Bokkee
Format: Article
Language:English
Subjects:
Antisense RNA
Chromatin
Chromosome 7
Chromosomes
Clusters
Developmental Biology
enhancer
Enhancers
Gene expression
Genomic imprinting
H19
Igf2
Igf2as
imprinting
Insulators
Insulin-like growth factor II
Muscles
Regulatory sequences
Transcription
생물학
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Summary:Igf2, H19, and Nctc1 are linked co-regulated genes on distal mouse chromosome 7. This locus is an important model both for studying mechanisms of monoallelic expression and for elucidating the role of cis-regulatory elements - enhancers and insulators - in organizing chromatin and gene expression across a large domain. In this study we characterize regulated expression of the Igf2 antisense transcript (Igf2as) in primary muscle cells. We demonstrate that Igf2as is imprinted (expressed only from the paternal chromosome). We also show that Igf2as expression during differentiation follows the same patterns as Igf2 and H19. Moreover, this expression is dependent upon the same shared enhancer element. Thus, our work shows that the imprinted cluster includes Igf2as in addition to H19, Igf2, and Nctc1.
ISSN:1976-8354
2151-2485
DOI:10.1080/19768354.2019.1612780