Dual‐color oligo‐FISH can reveal chromosomal variations and evolution in Oryza species

Summary Fluorescence in situ hybridization using probes based on oligonucleotides (oligo‐FISH) is a useful tool for chromosome identification and karyotype analysis. Here we developed two oligo‐FISH probes that allow the identification of each of the 12 pairs of chromosomes in rice (Oryza sativa). T...

Full description

Saved in:
Bibliographic Details
Published in:The Plant journal : for cell and molecular biology 2020-01, Vol.101 (1), p.112-121
Main Authors: Liu, Xiaoyu, Sun, Shang, Wu, Ying, Zhou, Yong, Gu, Siwei, Yu, Hengxiu, Yi, Chuandeng, Gu, Minghong, Jiang, Jiming, Liu, Bao, Zhang, Tao, Gong, Zhiyun
Format: Article
Language:English
Subjects:
Abnormalities
Biological evolution
chromosomal variation
Chromosome aberrations
Chromosomes
Color
DNA probes
dual‐color oligo‐FISH
Fluorescence
Fluorescence in situ hybridization
Genomes
karyotype
Karyotypes
Meiosis
Nucleotides
Oligonucleotides
Oryza
Oryza sativa
Oryza species
Probes
Rice
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Summary Fluorescence in situ hybridization using probes based on oligonucleotides (oligo‐FISH) is a useful tool for chromosome identification and karyotype analysis. Here we developed two oligo‐FISH probes that allow the identification of each of the 12 pairs of chromosomes in rice (Oryza sativa). These two probes comprised 25 717 (green) and 25 215 (red) oligos (45 nucleotides), respectively, and generated 26 distinct FISH signals that can be used as a barcode to uniquely label each of the 12 pairs of rice chromosomes. Standard karyotypes of rice were established using this system on both mitotic and meiotic chromosomes. Moreover, dual‐color oligo‐FISH was used to characterize diverse chromosomal abnormalities. Oligo‐FISH analyses using these probes in various wild Oryza species revealed that chromosomes from the AA, BB or CC genomes generated specific and intense signals similar to those in rice, while chromosomes with the EE genome generated less specific signals and the FF genome gave no signal. Together, the oligo‐FISH probes we established will be a powerful tool for studying chromosome variations and evolution in the genus Oryza. Significance Statement Dual‐color oligo‐FISH was established to allow identification of the 12 chromosomes in mitosis and meiosis of rice, which will be a useful tool for studying chromosome variations and evolution in the Oryza genus.
ISSN:0960-7412
1365-313X
DOI:10.1111/tpj.14522