Synthesis, characterization, in vitro biocompatibility and antibacterial properties study of nanocomposite materials based on hydroxyapatite-biphasic ZnO micro- and nanoparticles embedded in Alginate matrix

• Published in: Materials Science & Engineering C 2019-11, Vol.104, p.109965-109965, Article 109965
• Main Authors: Turlybekuly, A., Pogrebnjak, A.D., Sukhodub, L.F., Sukhodub, L.B., Kistaubayeva, A.S., Savitskaya, I.S., Shokatayeva, D.H., Bondar, O.V., Shaimardanov, Zh.K., Plotnikov, S.V., Shaimardanova, B.H., Digel, I.
• Format: Article
• Language: English
• Subjects: Alginates - chemistry; Alginic acid; Ammonia; Ammonium; Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Antibacterial properties of HA-Alg-ZnO; Antimicrobial activity; Bacteria - drug effects; Beads; Biocompatibility; Biomimetics; Calcium; Calcium chloride; Calcium ions; Calcium nitrate; Cell Line; Coated Materials, Biocompatible - chemistry; Crystals; Cytotoxicity; Durapatite - chemistry; E coli; Embedding; Gram-positive bacteria; Hydrogels - chemistry; Hydrogels - pharmacology; Hydroxyapatite; Hydroxyapatite on alginate matrix; Incubation; Ions; Lattices; Materials science; Mice; Multiphase; Nanocomposites; Nanocomposites - chemistry; Nanocrystals; Nanoparticles; Nanoparticles - chemistry; Nanostructured biocomposite; NIH 3T3 Cells; Phase transitions; Properties (attributes); Radiation; Sodium; Sodium alginate; Synthesis; Toxicity; Zinc; Zinc oxide; Zinc Oxide - chemistry; Zinc oxides; Zn doped biocomposite; Zn doped hydroxyapatite biocompatibility; ZnO nanoparticles
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2019.109965

The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride. The samples' antimicrobial activity assessment was carried out on Gram-negative (E. coli, P. aeruginosa) and Gram-positive bacteria (S. aureus and S. epidermidis) test cultures by the co-incubation and modified “agar diffusion” methods. The murine fibroblast cells were used for the biocompatibility tests and cytotoxicity evaluation. It was shown that synthesized nanocomposite material has a multiphase nanoscale architecture, where ZnO nanocrystals are represented by two lattices: cubic and hexagonal. The possible explanation of ZnO nanocrystals' phase transition is given. At the same time, a partial replacement of Ca2+ ions by Zn2+ ions in the HA lattice possibly occurs due to processing of composite by US radiation. The replacement was evidenced by the non-stoichiometric Ca/P ratio