Down-regulation effects of IFN-α on p11, 5-htr1b and 5-HTR4 protein levels were affected by NH4CL or MG132 treatment in SH-sy5y cells

• Published in: Journal of biosciences 2019-09, Vol.44 (4), p.1-10, Article 101
• Main Authors: Guo, Jiqiang, Ding, Huaxia, Lv, Zhuangwei, Jiao, Junna, Wang, Hui, Ji, Yanhong
• Format: Article
• Language: English
• Subjects: Ammonium chloride; Biomedical and Life Sciences; Biomedicine; Cell Biology; Cells; Down-regulation; Interferon; Levels; Life Sciences; Lysosomes; Microbiology; mRNA; Nucleic acids; Plant Sciences; Post-transcription; Proteasomes; Proteins; RNA; Serotonin; Serotonin S1 receptors; Serotonin S4 receptors; Ubiquitin; Ubiquitination; Western blotting; Zoology; α-Interferon
• ISSN: 0250-5991; 0973-7138
• DOI: 10.1007/s12038-019-9906-2

In previous studies, we found interferon-α (IFN-α) could reduce protein levels of p11, 5-hydroxytryptamine receptor 1b (5-HT1b) and 5-hydroxytryptamine receptor 4 (5-HT4), but does not influence their messenger RNA levels in SH-sy5y cells. Thus, we investigated the post-transcriptional modulation of these molecules by IFN-α. SH-sy5y cells were treated with IFN-α, NH 4 Cl or MG132 alone or in combination, and then the protein levels of p11, 5-HT1b and 5-HT4 were analyzed by western blots. The regulatory effects of p11 on 5-HT1b and 5-HT4 were also determined in p11 knock-down cells. NH 4 Cl but not MG132 could reverse the protein level of p11 in IFN-α-treated SH-sy5y cells. MG132 could recover the protein levels of 5-HT1b and 5-HT4 in p11 knock-down cells. The down-regulation effects of IFN-α on p11, 5-HT1b and 5-HT4 were associated with the lysosome and ubiquitin–proteasome-mediated pathways. p11 was identified as a potent regulator to modulate the ubiquitination of 5-HT1b and 5-HT4. Therefore, it could be potential target therapies in IFN-α-induced depression.