Volume-CLEM: a method for correlative light and electron microscopy in three dimensions

Generation of three-dimensional (3D) data sets from serial sections of tissues imaged by light microscopy (LM) allows identification of rare structures by morphology or fluorescent labeling. Here, we demonstrate a workflow for correlative LM and electron microscopy (EM) from 3D LM to 3D EM, using th...

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Bibliographic Details
Published in:American journal of physiology. Lung cellular and molecular physiology 2019-12, Vol.317 (6), p.L778-L784
Main Authors: Hegermann, Jan, Wrede, Christoph, Fassbender, Susanne, Schliep, Ronja, Ochs, Matthias, Knudsen, Lars, Mühlfeld, Christian
Format: Article
Language:English
Subjects:
Animals
Electron microscopy
Fluorescence
Imaging, Three-Dimensional - methods
Light microscopy
Lung - ultrastructure
Mice
Mice, Inbred C57BL
Microscopy
Microscopy, Electron, Scanning - methods
Microscopy, Fluorescence - methods
Morphology
Optical microscopy
Organs
Workflow
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Description
Summary:Generation of three-dimensional (3D) data sets from serial sections of tissues imaged by light microscopy (LM) allows identification of rare structures by morphology or fluorescent labeling. Here, we demonstrate a workflow for correlative LM and electron microscopy (EM) from 3D LM to 3D EM, using the same sectioned material for both methods consecutively. The new approach is easy to reproduce in routine EM laboratories and applicable to a wide range of organs and research questions.
ISSN:1040-0605
1522-1504
DOI:10.1152/ajplung.00333.2019