Aminoantipyrine based efficient chemosensor for Zn(II) ions and its effectiveness in live cell imaging

• Published in: Journal of photochemistry and photobiology. B, Biology Biology, 2019-10, Vol.199, p.111602-111602, Article 111602
• Main Authors: Wang, Yu, Xia, Chunhui, Han, Zhendong, Jiao, Yv, Yao, Xu, Lun, Zhiqiang, Fu, Shuang, Zhang, Hongguang, Hou, Peng, Ning, Deli
• Format: Article
• Language: English
• Subjects: Ampyrone - chemistry; Biosensing Techniques - methods; Cadmium; Cadmium - analysis; Cations, Divalent - analysis; Cell Survival - drug effects; Cell walls; Charge transfer; Chemical sensors; Chemoreceptors; Colorimetry; Cytotoxicity; Fluorescence; Fluorescence spectroscopy; Fluorescent Dyes - chemistry; HeLa Cells; Humans; Imaging; Internal charge transfer; Mercury; Mercury - analysis; Mercury compounds; Metal ions; Molecular Structure; Optical Imaging - methods; Sensitivity and Specificity; Sensor; Structure-Activity Relationship; Toxicity; Zinc; Zinc - analysis; Zinc(II)
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/j.jphotobiol.2019.111602

A simple imine based receptor NA-1 has been synthesized for detection of Zinc ions. Probe NA-1 showed the selective colorimetric changes with Zinc (II) ions whereas other metal ions didn't showed any observable colorimetric changes. The probe showed the very selective turn-on fluorescence response with Zn(II) ions among other rival metal ions like Cd(II) and Hg(II). The mode of binding was studied by 1H-nmr titrations and fluorescence spectroscopy. Jobs plot analysis confirming that the probe NA-1 forms 1:1 complex with Zn(II). The observed fluorescence and absorption change further supported by theoretical calculations. The turn-on fluorescence of the probe NA-1 is probably attributable to the interruption of intramolecular charge transfer as well as ESIPT. The limit of detection of the probe for Zn(II) sensing is in the range of 14 nano molar. Cytotoxicity (MTT) assay of the probe in live HeLa cells is showing that the probe is least toxic to cells. The probe NA-1 is effectively applied to detect Zn(II) ions in HeLa cells and suggesting the probe is NA-l permeable to cell wall and viable for Zinc(II) ions imaging in live cells. •Simple imine based sensor for Zinc(II) ions•Colorimetric and turn-on fluorescence sensor•Internal charge transfer process•Applicable for live cell imaging