Seroprevalence and molecular characterization of Brucella species in naturally infected cattle and sheep

•Brucellosis remains a threat to the cattle and sheep industry in Egypt.•Detection of B. abortus from unvaccinated cattle and sheep.•PCR is a better alternative to bacteriological methods for diagnosing brucellosis.•Sequencing and phylogenetic analyses showed that two distinct B. abortus strains are...

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Bibliographic Details
Published in:Preventive veterinary medicine 2019-11, Vol.171, p.104756-104756, Article 104756
Main Authors: Selim, Abdelfattah, Attia, Kotb, Ramadan, Eman, Hafez, Yaser M., Salman, Alamery
Format: Article
Language:English
Subjects:
Animals
Brucella abortus - genetics
Brucella abortus - isolation & purification
Brucellosis
Brucellosis - blood
Brucellosis - epidemiology
Brucellosis - veterinary
Brucellosis, Bovine
Cattle
Databases, Nucleic Acid
Egypt - epidemiology
ELISA
Female
Male
PCR
Polymerase Chain Reaction
Risk Factors
Seroepidemiologic Studies
Seroprevalence
Sheep
Sheep Diseases - blood
Sheep Diseases - epidemiology
Sheep Diseases - microbiology
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Summary:•Brucellosis remains a threat to the cattle and sheep industry in Egypt.•Detection of B. abortus from unvaccinated cattle and sheep.•PCR is a better alternative to bacteriological methods for diagnosing brucellosis.•Sequencing and phylogenetic analyses showed that two distinct B. abortus strains are circulating among cattle and sheep.•Sex and age are considered as risk factor for brucellosis in cattle and sheep. Brucellosis is one of the most economically significant infectious diseases in Egypt. The study aimed to assess the seroprevalence by indirect ELISA by examining serum samples from 720 cattle and 320 sheep and detection and characterization of Brucella spp. from 24 clinical samples (placenta) by bacterial culture (BC) and PCR targeting bcsp31 gene. The seroprevalence was 16.7% and 16.25% in cattle and sheep, respectively. There was a significant association (P  2 years. Likewise, seroprevalence was significantly (P 
ISSN:0167-5877
1873-1716
DOI:10.1016/j.prevetmed.2019.104756