‘Ready Mixed’, improved nucleic acid amplification assays for the detection of Escherichia coli DNA and RNA

The selective amplification of E. coli nucleic acid sequences could be used for the early warning of faecal contamination in environmental samples. Modified assays for E. coli DNA and RNA markers are presented with improved integrity and performance over existing methods, and demonstrated using ‘rea...

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Bibliographic Details
Published in:Journal of microbiological methods 2019-10, Vol.165, p.105721-105721, Article 105721
Main Authors: McQuillan, Jonathan S., Wilson, Matthew W.
Format: Article
Language:English
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Summary:The selective amplification of E. coli nucleic acid sequences could be used for the early warning of faecal contamination in environmental samples. Modified assays for E. coli DNA and RNA markers are presented with improved integrity and performance over existing methods, and demonstrated using ‘ready mixed’, preserved reagent mixtures.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2019.105721