Genetic diversity of groEL and msp4 sequences of Anaplasma ovis infecting camels from Tunisia

To date, no information is available regarding the infection of camels (Camelus dromedarius) by Anaplasma ovis in North African region. Several animal species can be infected by A. ovis which further complicates its natural infection cycle. In this paper, we investigated the occurrence and the genet...

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Bibliographic Details
Published in:Parasitology international 2020-02, Vol.74, p.101980-101980, Article 101980
Main Authors: Selmi, Rachid, Ben Said, Mourad, Dhibi, Mokhtar, Ben Yahia, Houcine, Abdelaali, Hedi, Messadi, Lilia
Format: Article
Language:English
Subjects:
Anaplasma - genetics
Anaplasma ovis
Anaplasmosis - blood
Anaplasmosis - epidemiology
Animals
Bacterial Proteins - genetics
Camels (Camelus dromedarius)
Camelus - microbiology
Chaperonin 60 - genetics
Female
Genetic Variation
Genotype
Male
Molecular detection
msp4 and groEL typing
Phylogenetic analysis
Phylogeny
RNA, Ribosomal, 16S - genetics
Tick Infestations - veterinary
Ticks - microbiology
Tunisia
Tunisia - epidemiology
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Summary:To date, no information is available regarding the infection of camels (Camelus dromedarius) by Anaplasma ovis in North African region. Several animal species can be infected by A. ovis which further complicates its natural infection cycle. In this paper, we investigated the occurrence and the genetic diversity of A. ovis in camels and ticks collected from them in Tunisia and the risk factor analysis. Camel blood samples (n = 412) and tick (n = 300) samples, identified as Hyalomma dromedarii (n = 149, 49.6%), H. impeltatum (n = 142, 47.3%) and H. excavatum (n = 9, 3%), were analyzed by conventional PCR followed by the sequencing of msp4 and groEL genes. A. ovis DNA was identified in five camels (1.2%), but not in infesting ticks (0%). The microscopic examination revealed the specific infection of camel erythrocytes by Anaplasma inclusions. The msp4 and groEL typing confirmed the natural infection of camels by A. ovis and revealed two different msp4 genotypes earlier detected in Tunisian small ruminants and their infested ticks, and five different and novel groEL genetic variants forming a separately sub-cluster within A. ovis cluster. The occurrence of different A. ovis strains specific to camels associated with a low prevalence of this Anaplasma species in camels may enrich knowledge regarding the distribution and the transmission cycle of this bacterium in arid and Saharan areas of Tunisia.
ISSN:1383-5769
1873-0329
DOI:10.1016/j.parint.2019.101980