Fe3O4@Au nanoparticles-based magnetoplatform for the HMGA maize endogenous gene electrochemical genosensing

This work addresses a technological advance applied to the construction of a magnetogenoassay with electrochemical transduction for the maize taxon-specific (HMGA gene) detection using gold-coated magnetic nanoparticles as nanosized platform. Superparamagnetic core-shell Fe3O4@Au nanoparticles (10.4...

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Published in:Talanta (Oxford) 2020-01, Vol.206, p.120220-120220, Article 120220
Main Authors: Sousa, Juliana Beatriz, Ramos-Jesus, Joilson, Silva, L.C., Pereira, C., de-los-Santos-Álvarez, N., Fonseca, Rosana A.S., Miranda-Castro, R., Delerue-Matos, C., Santos Júnior, J. Ribeiro, Barroso, M. Fátima
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Language:English
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Electrochemical genosensors
Fe3O4@Au magnetic nanoparticles
GMO
HMGA gene
Maize
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cited_by cdi_FETCH-LOGICAL-c389t-7d8451228ab4963ea797531da3f211a1e51e2d3a338951a4ae3548884dbbe8123
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container_title Talanta (Oxford)
container_volume 206
creator Sousa, Juliana Beatriz
Ramos-Jesus, Joilson
Silva, L.C.
Pereira, C.
de-los-Santos-Álvarez, N.
Fonseca, Rosana A.S.
Miranda-Castro, R.
Delerue-Matos, C.
Santos Júnior, J. Ribeiro
Barroso, M. Fátima
description This work addresses a technological advance applied to the construction of a magnetogenoassay with electrochemical transduction for the maize taxon-specific (HMGA gene) detection using gold-coated magnetic nanoparticles as nanosized platform. Superparamagnetic core-shell Fe3O4@Au nanoparticles (10.4 ± 1.7 nm) were used to assemble the genoassay through the covalent immobilization of HMGA DNA probes onto carboxylated self-assembled monolayers at the nanoparticles surface. A hybridization reaction using sandwich format was selected to prevent inefficient hybridization connected with stable secondary DNA structures using also fluorescein isothiocyanate as DNA signaling tag. The labelling of the hybridization reaction with enzymes allowed the chronoamperometric measurement of the peroxidase activity linked to the nanoplatform located on gold surface. Using this electrochemical magnetogenoassay a linear concentration range from 0.5 to 5 nM and a LOD of 90 pM with a RSD
doi_str_mv 10.1016/j.talanta.2019.120220
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subjects Electrochemical genosensors
Fe3O4@Au magnetic nanoparticles
GMO
HMGA gene
Maize
title Fe3O4@Au nanoparticles-based magnetoplatform for the HMGA maize endogenous gene electrochemical genosensing
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