Induction of immediate early genes expression in the mouse striatum following acute administration of synthetic cathinones

• Published in: Pharmacological reports 2019-12, Vol.71 (6), p.977-982
• Main Authors: Wojcieszak, Jakub, Andrzejczak, Dariusz, Szymańska, Bożena, Zawilska, Jolanta B.
• Format: Article
• Language: English
• Subjects: Addiction; Drug Safety and Pharmacovigilance; Immediate early gene expression; MDPV; Pharmacotherapy; Pharmacy; Short Communication; Synthetic cathinones; α-PVP
• ISSN: 1734-1140; 2299-5684
• DOI: 10.1016/j.pharep.2019.05.011

•Synthetic cathinones increase expression of immediate early genes in the mouse striatum.•Up-regulated genes include Areg, Csrnp1, Dusp1, Dusp14, Egr2, Egr4, c-fos, FosB, Homer1, c-jun.•Synthetic cathinones could trigger neuroplastic processes underlying addition. Synthetic cathinones (SCs) form one of the most prominent group of the New Psychoactive Substances. SCs enhance central dopaminergic and noradrenergic neurotransmission, and are used as substitutes for illicit psychostimulants, namely cocaine, amphetamine, and methamphetamine. Changes in the expression of immediate early genes (IEGs) in the striatum underlie the addictive potential of drugs of abuse belonging to distinct pharmacologic groups. This work was aimed to assess the impact of acute administration of the prominent SCs on the mRNA levels of IEGs in the mouse striatum. Effects of 3,4-MDPV, 2,3-MDPV, α-PVP, PV8, PV9, methcathinone (MC) and 3-fluoromethcathinone (3-FMC) on the mRNA levels of ten IEGs, one and two hours after exposure, were measured in the mouse striatum using the quantitative RT-PCR technique. All SCs used in the study produced increased mRNA levels of the following IEGs: Areg, c-fos, Csrnp1, Dusp1, Dusp14, Egr2, Egr4 and FosB. Additionally, the majority of SCs increased the expression of Homer1 and c-jun. The magnitude of observed changes varied by the drug, analyzed gene and, in many cases, by time after administration. This study demonstrates that SCs increase the expression of IEGs in the mouse striatum, which may lead to a plethora of effects, as proteins encoded by the analyzed genes are involved in diverse actions, including an acute response to the drug and the neuroplasticity underlying the development of addiction.