Overexpression of lncRNA Gm2691 attenuates apoptosis and inflammatory response after myocardial infarction through PI3K/Akt signaling pathway

• Published in: IUBMB life 2019-10, Vol.71 (10), p.1561-1570
• Main Authors: Li, Tingting, Tian, Hongbo, Li, Jun, Zuo, Anju, Chen, Jiying, Xu, Dan, Guo, Yuan, Gao, Haiqing
• Format: Article
• Language: English
• Subjects: 1-Phosphatidylinositol 3-kinase; AKT protein; Animals; Apoptosis; Apoptosis - genetics; Cardiomyocytes; Cell survival; Gene Expression Regulation; Gm2691; Heart attacks; Humans; Inflammation; Inflammation - genetics; Inflammation - pathology; inflammation response; Ischemia; ischemic reperfusion injury; Myocardial infarction; Myocardial Infarction - genetics; Myocardial Infarction - pathology; Myocardial Reperfusion Injury - genetics; Myocardial Reperfusion Injury - pathology; Myocytes, Cardiac - metabolism; Myocytes, Cardiac - pathology; Neonates; Phosphatidylinositol 3-Kinases - genetics; Proto-Oncogene Proteins c-akt - genetics; Rats; Reperfusion; RNA, Long Noncoding - genetics; Signal transduction; Signal Transduction - genetics; Ventricle
• ISSN: 1521-6543; 1521-6551
• DOI: 10.1002/iub.2081

Acute myocardial infarction is one of the most threatening disease in the world. In previous studies, numerous dysregulated lncRNAs exposed to ischemic reperfusion injury have been identified. In this differential lncRNAs, Gm2691 attracted our attention due to its high fold change. The aim of the study was to investigate the function and mechanism of lncRNA Gm2691 in ischemic reperfusion injury. AnaeroPack anaerobic system treated neonatal rat ventricular cardiomyocytes were used to analyze the function of lncRNA Gm2691 in vitro. Tunel, Caspase3, and inflammation markers were detected to evaluate apoptosis and inflammatory response. Rat acute myocardial infarction was performed to elucidate the function of lncRNA Gm2691 in vivo. The results showed that LncRNA Gm2691 improved the cardiac function and attenuated the inflammatory response in vivo. We also found that lncRNA Gm2691 reduced the apoptosis and improved cell survival rates in anaeroPack anaerobic system treated neonatal rat ventricular cardiomyocytes. Western blot analysis revealed that lncRNA Gm2691 decreased Akt and ERK1/2 activities, suggesting that lncRNA Gm2691 may functioned through Akt signaling pathway. We verified the function and mechanism of lncRNA Gm2691 and provide evidence that lncRNA Gm2691 may play important role in ischemic reperfusion injury, and understanding the precise role of Gm2691 will undoubtedly shed new light on the clinical treatment.