Identification of duplicated suppressor of cytokine signaling 3 (SOCS3) genes in blunt snout bream (Megalobrama amblycephala)

• Published in: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2020-01, Vol.239, p.110348-110348, Article 110348
• Main Authors: Zhao, Shan-shan, Zhao, Xin-yu, Wu, Cheng-bin, Zheng, Guo-dong, Zou, Shu-ming
• Format: Article
• Language: English
• Subjects: Aerononas hydrophila; Animals; Cyprinidae - metabolism; Expression analysis; Fish Proteins - biosynthesis; Gene Expression Regulation - physiology; hGH; Megalobrama amblycephala; Organ Specificity; socs3; Suppressor of Cytokine Signaling 3 Protein - biosynthesis
• ISSN: 1096-4959; 1879-1107
• DOI: 10.1016/j.cbpb.2019.110348

The suppressor of cytokine signaling 3 (SOCS3) negatively regulates the responses of various immune cytokines. In this study, we identified socs3s genes of blunt snout bream. 209- and 216-aa long peptides are encoded by socs3a and socs3b genes, respectively. The socs3s mRNAs are expressed consistently during the entire process of embryonic development. Whole-mount in situ hybridization detected socs3a in the eyes and posterior somites at 12 h post fertilization (hpf), transcribed at the otic vesicle at 24 hpf, and transcribed at the eyes, brain, and otic vesicle at 36 hpf; while the socs3b mRNA was transcribed at the notochord at 12 hpf, expressed in the brain, eyes, and tailbud at 24 hpf, and detected in the brain at 36 hpf. The expression of socs3a is slightly different from that of socs3b in tissues of juvenile and adult blunt snout bream. After recombinant human growth hormone (hGH) treatment, the transcript levels of socs3s of blunt snout bream were increased in gills, spleen, kidney, and gonads. After Aerononas hydrophila infection, the mRNA levels of socs3s of blunt snout bream were significantly increased in the liver, spleen, intestine, and kidney tissues. Blunt snout bream were susceptible to various pathogenic microorganisms, we intraperitoneally injected blunt snout bream with A. hydrophila to explore the immune mechanism of socs3s. These results suggested that socs3s of blunt snout bream plays important roles in the regulation of embryonic development and tissue growth, and that socs3s may also play key roles in regulating the bacterial-induced congenital immune response. Socs3s genes has the potential to be used as targeted genes to improve the immunity against bacteria, which is conducive to the improvement of production and breeding. [Display omitted] •Two distinct socs3 genes has the potential to be used as targeted genes to improve the immunity against bacteria.