Two new species of Gloniopsis (Hysteriales, Ascomycota) from clinical specimens: Morphological and molecular characterisation

• Published in: Mycoses 2019-12, Vol.62 (12), p.1164-1173
• Main Authors: Valenzuela‐Lopez, Nicomedes, Magaña‐Dueñas, Viridiana, Cano‐Lira, José F., Wiederhold, Nathan, Guarro, Josep, Stchigel, Alberto M.
• Format: Article
• Language: English
• Subjects: Ascomycota; Ascomycota - classification; Ascomycota - isolation & purification; coelomycetes; Conidia; DNA, Fungal - genetics; DNA, Ribosomal Spacer - genetics; Fruit bodies; Gloniopsis; Humans; Hyphae; Hysteriales; Morphology; Mycoses - microbiology; mycosis; New species; Phylogeny; Pycnidia; Rhytidhysteron; RNA, Ribosomal, 28S - genetics; rRNA 28S; Sequence Analysis, DNA; Spacer region; Spores, Fungal; Translation elongation
• ISSN: 0933-7407; 1439-0507
• DOI: 10.1111/myc.13006

Summary Background The coelomycetes comprise a wide range of fungal species distributed in at least three different classes of the phylum Ascomycota. These are morphologically characterised by producing their conidia inside of fruiting bodies called pycnidia or acervuli, and only a reduced number of species are able to cause human infections. However, their identification in the clinical laboratory is often difficult, due to their few morphological features or because they remain sterile. Materials and Methods In the present study, three isolates of coelomycetes of clinical origin were phenotypically and molecularly studied, by sequencing the D1‐D2 fragment of the 28S nuclear ribosomal RNA (nrRNA) (LSU), the internal transcribed spacer region (ITS1‐5.8S‐ITS2) and a fragment of the translation elongation factor 1‐alpha (tef1) genes. Results and Conclusions As result of the molecular analysis, the isolates were identified as belonging to the genus Gloniopsis (order Hysteriales, Dothideomycetes) but without the characteristics of any of the species described so far. Therefore, we propose the new species Gloniopsis percutanea and Gloniopsis pneumoniae. Furthermore, this study revealed that some isolates from clinical specimens identified previously as Rhytidhysteron spp. were misidentified, and considering the few studies in the order Hysteriales and the scarce number of sequences of phylogenetic markers, future revisions of this order should be performed to clarify their taxonomy and obtain a better identification from isolates involved in human mycoses.