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Testing the efficacy of kitasamycin for use in the control and treatment of swine dysentery in experimentally infected pigs
Background Swine dysentery (SD) caused by Brachyspira hyodysenteriae is an important disease in Australia. Aim The aim of this study is to evaluate the macrolide antibiotic kitasamycin for use in SD control. Methods The minimum inhibitory concentrations (MICs) of kitasamycin, tylosin and lincomycin...
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| Published in: | Australian veterinary journal 2019-11, Vol.97 (11), p.452-464 |
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| Main Authors: | , , , , , |
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| cites | cdi_FETCH-LOGICAL-c3536-a882b4b91b7a05b1c4c50571835e3c271159a5640195efc19951471cf0d7a1533 |
| container_end_page | 464 |
| container_issue | 11 |
| container_start_page | 452 |
| container_title | Australian veterinary journal |
| container_volume | 97 |
| creator | La, T Phillips, ND Dunlop, H Lugsomya, K Coiacetto, F Hampson, DJ |
| description | Background
Swine dysentery (SD) caused by Brachyspira hyodysenteriae is an important disease in Australia.
Aim
The aim of this study is to evaluate the macrolide antibiotic kitasamycin for use in SD control.
Methods
The minimum inhibitory concentrations (MICs) of kitasamycin, tylosin and lincomycin for 32 Australian isolates of B. hyodysenteriae were evaluated. Mutations in the 23S rRNA gene were examined. Isolate ‘13’ with a low kitasamycin MIC was used to challenge weaner pigs. Sixty pigs were housed in 20 pens each containing three pigs: pigs in four pens received 2 kg/tonne of a product containing kitasamycin (3.1% active) prophylactically in their food starting 4 days before B. hyodysenteriae challenge (group 1); pigs in four pens were challenged and received the same dose therapeutically once one pig in a pen showed diarrhoea (group 2); four pens were challenged and received 4 kg/tonne of the product therapeutically (group 3); four pens were challenged but not medicated (group 4); two pens were unmedicated and unchallenged (group 5) and two pens received 2 kg/tonne and were unchallenged (group 6). Pigs were monitored for B. hyodysenteriae excretion and disease.
Results
Macrolide resistance was widespread, and mutations in the 23S rRNA gene were identified in 23 isolates. Four isolates with kitasamycin MICs |
| doi_str_mv | 10.1111/avj.12876 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2293005431</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2293005431</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3536-a882b4b91b7a05b1c4c50571835e3c271159a5640195efc19951471cf0d7a1533</originalsourceid><addsrcrecordid>eNp10U1v1DAQBmCrArVL4dA_UFniUg5pZ-x4nRyrqnypEpfC1XKccfGSTbZ20hLx53HYlgMSvvhDj195PIydIJxjHhf2YXOOotLrA7ZCrbCACuULtgIAVUAp5BF7ldIGQGol1CE7kqhEXWpYsV-3lMbQ3_HxO3HyPjjrZj54_iOMNtnt7ELP_RD5lIjn5cLc0I9x6LjtWz5GsuOW-nG5kx5DT7ydU95TnBdPP3cUwwJs1y0nntxILd-Fu_SavfS2S_TmaT5mX99f3159LG6-fPh0dXlTOKnkurBVJZqyqbHRFlSDrnQKlMZKKpJOaERVW7UuAWtF3mFdKyw1Og-ttqikPGZn-9xdHO6nXK_ZhuSo62xPw5SMELXMP1VKzPTtP3QzTLHPrzNCQlXKCkBk9W6vXBxSiuTNLtdo42wQzNIRkzti_nQk29OnxKnZUvtXPrcgg4s9eAwdzf9PMpffPu8jfwP86pTc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2308438002</pqid></control><display><type>article</type><title>Testing the efficacy of kitasamycin for use in the control and treatment of swine dysentery in experimentally infected pigs</title><source>Wiley</source><creator>La, T ; Phillips, ND ; Dunlop, H ; Lugsomya, K ; Coiacetto, F ; Hampson, DJ</creator><creatorcontrib>La, T ; Phillips, ND ; Dunlop, H ; Lugsomya, K ; Coiacetto, F ; Hampson, DJ</creatorcontrib><description>Background
Swine dysentery (SD) caused by Brachyspira hyodysenteriae is an important disease in Australia.
Aim
The aim of this study is to evaluate the macrolide antibiotic kitasamycin for use in SD control.
Methods
The minimum inhibitory concentrations (MICs) of kitasamycin, tylosin and lincomycin for 32 Australian isolates of B. hyodysenteriae were evaluated. Mutations in the 23S rRNA gene were examined. Isolate ‘13’ with a low kitasamycin MIC was used to challenge weaner pigs. Sixty pigs were housed in 20 pens each containing three pigs: pigs in four pens received 2 kg/tonne of a product containing kitasamycin (3.1% active) prophylactically in their food starting 4 days before B. hyodysenteriae challenge (group 1); pigs in four pens were challenged and received the same dose therapeutically once one pig in a pen showed diarrhoea (group 2); four pens were challenged and received 4 kg/tonne of the product therapeutically (group 3); four pens were challenged but not medicated (group 4); two pens were unmedicated and unchallenged (group 5) and two pens received 2 kg/tonne and were unchallenged (group 6). Pigs were monitored for B. hyodysenteriae excretion and disease.
Results
Macrolide resistance was widespread, and mutations in the 23S rRNA gene were identified in 23 isolates. Four isolates with kitasamycin MICs < 5 μg/mL were considered susceptible. Following experimental challenge, 10 of 12 unmedicated pigs developed SD. No pigs receiving kitasamycin prophylactical or therapeutically developed SD. Medicated pigs shed low numbers of B. hyodysenteriae in their faeces.
Conclusions
Kitasamycin can help control SD in pigs infected with susceptible isolates of B. hyodysenteriae.</description><identifier>ISSN: 0005-0423</identifier><identifier>EISSN: 1751-0813</identifier><identifier>DOI: 10.1111/avj.12876</identifier><identifier>PMID: 31529470</identifier><language>eng</language><publisher>Melbourne: Wiley Publishing Asia Pty Ltd</publisher><subject>Animals ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Antimicrobial agents ; antimicrobial resistance ; Autopsy - veterinary ; Brachyspira hyodysenteriae ; Brachyspira hyodysenteriae - drug effects ; Diarrhea ; Disease Models, Animal ; Disease resistance ; Dysentery ; Dysentery, Bacillary - drug therapy ; Dysentery, Bacillary - microbiology ; Dysentery, Bacillary - pathology ; Dysentery, Bacillary - veterinary ; Genes, rRNA - drug effects ; Gram-Negative Bacterial Infections - drug therapy ; Gram-Negative Bacterial Infections - pathology ; Gram-Negative Bacterial Infections - veterinary ; Hogs ; kitasamycin ; Kitasamycin - pharmacology ; Lincomycin ; Livestock ; macrolide ; Male ; Microbial Sensitivity Tests ; Minimum inhibitory concentration ; Mutation ; rRNA ; rRNA 23S ; Sequence Analysis, RNA ; Swine ; Swine Diseases - drug therapy ; Swine Diseases - pathology ; Swine dysentery ; Tylosin ; Waterborne diseases ; Western Australia</subject><ispartof>Australian veterinary journal, 2019-11, Vol.97 (11), p.452-464</ispartof><rights>2019 Australian Veterinary Association</rights><rights>2019 Australian Veterinary Association.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3536-a882b4b91b7a05b1c4c50571835e3c271159a5640195efc19951471cf0d7a1533</citedby><cites>FETCH-LOGICAL-c3536-a882b4b91b7a05b1c4c50571835e3c271159a5640195efc19951471cf0d7a1533</cites><orcidid>0000-0002-7729-0427</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31529470$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>La, T</creatorcontrib><creatorcontrib>Phillips, ND</creatorcontrib><creatorcontrib>Dunlop, H</creatorcontrib><creatorcontrib>Lugsomya, K</creatorcontrib><creatorcontrib>Coiacetto, F</creatorcontrib><creatorcontrib>Hampson, DJ</creatorcontrib><title>Testing the efficacy of kitasamycin for use in the control and treatment of swine dysentery in experimentally infected pigs</title><title>Australian veterinary journal</title><addtitle>Aust Vet J</addtitle><description>Background
Swine dysentery (SD) caused by Brachyspira hyodysenteriae is an important disease in Australia.
Aim
The aim of this study is to evaluate the macrolide antibiotic kitasamycin for use in SD control.
Methods
The minimum inhibitory concentrations (MICs) of kitasamycin, tylosin and lincomycin for 32 Australian isolates of B. hyodysenteriae were evaluated. Mutations in the 23S rRNA gene were examined. Isolate ‘13’ with a low kitasamycin MIC was used to challenge weaner pigs. Sixty pigs were housed in 20 pens each containing three pigs: pigs in four pens received 2 kg/tonne of a product containing kitasamycin (3.1% active) prophylactically in their food starting 4 days before B. hyodysenteriae challenge (group 1); pigs in four pens were challenged and received the same dose therapeutically once one pig in a pen showed diarrhoea (group 2); four pens were challenged and received 4 kg/tonne of the product therapeutically (group 3); four pens were challenged but not medicated (group 4); two pens were unmedicated and unchallenged (group 5) and two pens received 2 kg/tonne and were unchallenged (group 6). Pigs were monitored for B. hyodysenteriae excretion and disease.
Results
Macrolide resistance was widespread, and mutations in the 23S rRNA gene were identified in 23 isolates. Four isolates with kitasamycin MICs < 5 μg/mL were considered susceptible. Following experimental challenge, 10 of 12 unmedicated pigs developed SD. No pigs receiving kitasamycin prophylactical or therapeutically developed SD. Medicated pigs shed low numbers of B. hyodysenteriae in their faeces.
Conclusions
Kitasamycin can help control SD in pigs infected with susceptible isolates of B. hyodysenteriae.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>antimicrobial resistance</subject><subject>Autopsy - veterinary</subject><subject>Brachyspira hyodysenteriae</subject><subject>Brachyspira hyodysenteriae - drug effects</subject><subject>Diarrhea</subject><subject>Disease Models, Animal</subject><subject>Disease resistance</subject><subject>Dysentery</subject><subject>Dysentery, Bacillary - drug therapy</subject><subject>Dysentery, Bacillary - microbiology</subject><subject>Dysentery, Bacillary - pathology</subject><subject>Dysentery, Bacillary - veterinary</subject><subject>Genes, rRNA - drug effects</subject><subject>Gram-Negative Bacterial Infections - drug therapy</subject><subject>Gram-Negative Bacterial Infections - pathology</subject><subject>Gram-Negative Bacterial Infections - veterinary</subject><subject>Hogs</subject><subject>kitasamycin</subject><subject>Kitasamycin - pharmacology</subject><subject>Lincomycin</subject><subject>Livestock</subject><subject>macrolide</subject><subject>Male</subject><subject>Microbial Sensitivity Tests</subject><subject>Minimum inhibitory concentration</subject><subject>Mutation</subject><subject>rRNA</subject><subject>rRNA 23S</subject><subject>Sequence Analysis, RNA</subject><subject>Swine</subject><subject>Swine Diseases - drug therapy</subject><subject>Swine Diseases - pathology</subject><subject>Swine dysentery</subject><subject>Tylosin</subject><subject>Waterborne diseases</subject><subject>Western Australia</subject><issn>0005-0423</issn><issn>1751-0813</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp10U1v1DAQBmCrArVL4dA_UFniUg5pZ-x4nRyrqnypEpfC1XKccfGSTbZ20hLx53HYlgMSvvhDj195PIydIJxjHhf2YXOOotLrA7ZCrbCACuULtgIAVUAp5BF7ldIGQGol1CE7kqhEXWpYsV-3lMbQ3_HxO3HyPjjrZj54_iOMNtnt7ELP_RD5lIjn5cLc0I9x6LjtWz5GsuOW-nG5kx5DT7ydU95TnBdPP3cUwwJs1y0nntxILd-Fu_SavfS2S_TmaT5mX99f3159LG6-fPh0dXlTOKnkurBVJZqyqbHRFlSDrnQKlMZKKpJOaERVW7UuAWtF3mFdKyw1Og-ttqikPGZn-9xdHO6nXK_ZhuSo62xPw5SMELXMP1VKzPTtP3QzTLHPrzNCQlXKCkBk9W6vXBxSiuTNLtdo42wQzNIRkzti_nQk29OnxKnZUvtXPrcgg4s9eAwdzf9PMpffPu8jfwP86pTc</recordid><startdate>201911</startdate><enddate>201911</enddate><creator>La, T</creator><creator>Phillips, ND</creator><creator>Dunlop, H</creator><creator>Lugsomya, K</creator><creator>Coiacetto, F</creator><creator>Hampson, DJ</creator><general>Wiley Publishing Asia Pty Ltd</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7729-0427</orcidid></search><sort><creationdate>201911</creationdate><title>Testing the efficacy of kitasamycin for use in the control and treatment of swine dysentery in experimentally infected pigs</title><author>La, T ; Phillips, ND ; Dunlop, H ; Lugsomya, K ; Coiacetto, F ; Hampson, DJ</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3536-a882b4b91b7a05b1c4c50571835e3c271159a5640195efc19951471cf0d7a1533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics</topic><topic>Antimicrobial agents</topic><topic>antimicrobial resistance</topic><topic>Autopsy - veterinary</topic><topic>Brachyspira hyodysenteriae</topic><topic>Brachyspira hyodysenteriae - drug effects</topic><topic>Diarrhea</topic><topic>Disease Models, Animal</topic><topic>Disease resistance</topic><topic>Dysentery</topic><topic>Dysentery, Bacillary - drug therapy</topic><topic>Dysentery, Bacillary - microbiology</topic><topic>Dysentery, Bacillary - pathology</topic><topic>Dysentery, Bacillary - veterinary</topic><topic>Genes, rRNA - drug effects</topic><topic>Gram-Negative Bacterial Infections - drug therapy</topic><topic>Gram-Negative Bacterial Infections - pathology</topic><topic>Gram-Negative Bacterial Infections - veterinary</topic><topic>Hogs</topic><topic>kitasamycin</topic><topic>Kitasamycin - pharmacology</topic><topic>Lincomycin</topic><topic>Livestock</topic><topic>macrolide</topic><topic>Male</topic><topic>Microbial Sensitivity Tests</topic><topic>Minimum inhibitory concentration</topic><topic>Mutation</topic><topic>rRNA</topic><topic>rRNA 23S</topic><topic>Sequence Analysis, RNA</topic><topic>Swine</topic><topic>Swine Diseases - drug therapy</topic><topic>Swine Diseases - pathology</topic><topic>Swine dysentery</topic><topic>Tylosin</topic><topic>Waterborne diseases</topic><topic>Western Australia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>La, T</creatorcontrib><creatorcontrib>Phillips, ND</creatorcontrib><creatorcontrib>Dunlop, H</creatorcontrib><creatorcontrib>Lugsomya, K</creatorcontrib><creatorcontrib>Coiacetto, F</creatorcontrib><creatorcontrib>Hampson, DJ</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Australian veterinary journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>La, T</au><au>Phillips, ND</au><au>Dunlop, H</au><au>Lugsomya, K</au><au>Coiacetto, F</au><au>Hampson, DJ</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Testing the efficacy of kitasamycin for use in the control and treatment of swine dysentery in experimentally infected pigs</atitle><jtitle>Australian veterinary journal</jtitle><addtitle>Aust Vet J</addtitle><date>2019-11</date><risdate>2019</risdate><volume>97</volume><issue>11</issue><spage>452</spage><epage>464</epage><pages>452-464</pages><issn>0005-0423</issn><eissn>1751-0813</eissn><abstract>Background
Swine dysentery (SD) caused by Brachyspira hyodysenteriae is an important disease in Australia.
Aim
The aim of this study is to evaluate the macrolide antibiotic kitasamycin for use in SD control.
Methods
The minimum inhibitory concentrations (MICs) of kitasamycin, tylosin and lincomycin for 32 Australian isolates of B. hyodysenteriae were evaluated. Mutations in the 23S rRNA gene were examined. Isolate ‘13’ with a low kitasamycin MIC was used to challenge weaner pigs. Sixty pigs were housed in 20 pens each containing three pigs: pigs in four pens received 2 kg/tonne of a product containing kitasamycin (3.1% active) prophylactically in their food starting 4 days before B. hyodysenteriae challenge (group 1); pigs in four pens were challenged and received the same dose therapeutically once one pig in a pen showed diarrhoea (group 2); four pens were challenged and received 4 kg/tonne of the product therapeutically (group 3); four pens were challenged but not medicated (group 4); two pens were unmedicated and unchallenged (group 5) and two pens received 2 kg/tonne and were unchallenged (group 6). Pigs were monitored for B. hyodysenteriae excretion and disease.
Results
Macrolide resistance was widespread, and mutations in the 23S rRNA gene were identified in 23 isolates. Four isolates with kitasamycin MICs < 5 μg/mL were considered susceptible. Following experimental challenge, 10 of 12 unmedicated pigs developed SD. No pigs receiving kitasamycin prophylactical or therapeutically developed SD. Medicated pigs shed low numbers of B. hyodysenteriae in their faeces.
Conclusions
Kitasamycin can help control SD in pigs infected with susceptible isolates of B. hyodysenteriae.</abstract><cop>Melbourne</cop><pub>Wiley Publishing Asia Pty Ltd</pub><pmid>31529470</pmid><doi>10.1111/avj.12876</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-7729-0427</orcidid></addata></record> |
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| ispartof | Australian veterinary journal, 2019-11, Vol.97 (11), p.452-464 |
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| source | Wiley |
| subjects | Animals Anti-Bacterial Agents - pharmacology Antibiotics Antimicrobial agents antimicrobial resistance Autopsy - veterinary Brachyspira hyodysenteriae Brachyspira hyodysenteriae - drug effects Diarrhea Disease Models, Animal Disease resistance Dysentery Dysentery, Bacillary - drug therapy Dysentery, Bacillary - microbiology Dysentery, Bacillary - pathology Dysentery, Bacillary - veterinary Genes, rRNA - drug effects Gram-Negative Bacterial Infections - drug therapy Gram-Negative Bacterial Infections - pathology Gram-Negative Bacterial Infections - veterinary Hogs kitasamycin Kitasamycin - pharmacology Lincomycin Livestock macrolide Male Microbial Sensitivity Tests Minimum inhibitory concentration Mutation rRNA rRNA 23S Sequence Analysis, RNA Swine Swine Diseases - drug therapy Swine Diseases - pathology Swine dysentery Tylosin Waterborne diseases Western Australia |
| title | Testing the efficacy of kitasamycin for use in the control and treatment of swine dysentery in experimentally infected pigs |
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