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Angiosuppressive effects of bio-fabricated silver nanoparticles synthesis using Clitoria ternatea flower: an in vitro and in vivo approach
Biosynthesis of silver nanoparticles (CTNP’s) by Clitoria ternatea flower in the aqueous extract was investigated. Synthesized nanoparticles were characterized by using UV–Visible spectroscopy, followed by DLS, Zeta potential, XRD, FTIR, SEM, and AFM. The biocompatibility nature of CTNP’s was determ...
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| Published in: | Journal of biological inorganic chemistry 2019-10, Vol.24 (7), p.1115-1126 |
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| container_title | Journal of biological inorganic chemistry |
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| creator | Srinivas, Balaji Kyathegowdanadoddi Shivamadhu, Madhu Chakkere Siddappaji, Kiran Kumar Krishnappa, Dharmappa Kattepura Jayarama, Shankar |
| description | Biosynthesis of silver nanoparticles (CTNP’s) by
Clitoria ternatea
flower in the aqueous extract was investigated. Synthesized nanoparticles were characterized by using UV–Visible spectroscopy, followed by DLS, Zeta potential, XRD, FTIR, SEM, and AFM. The biocompatibility nature of CTNP’s was determined using erythrocytes model system. Cytotoxicity of CTNP’s against MCF-7 and EAC cells were determined by using MTT and Trypan blue exclusion method and their IC
50
was found to be 19.37 µg/mL and 24 µg/mL. Cytotoxic potential of CTNP’s was further confirmed by clonogenic assay. Further in vivo studies using EAC mice model supports the anti-cancer potential of silver nanoparticles. Results found that the CTNP’s effectively control the proliferation rate by inhibiting the ascites secretion and cellular density. Further quantification of VEGF, microvessel density counts and CAM assays show the anti-angiogenic potential of the CTNP’s. The apoptotic inducing activity of CTNP’s was confirmed by DNA fragmentation, fluorescent staining studies. More interestingly, EAC treated mice exhibit significant increase in lifespan (~ 2.25 fold) compared to control EAC mice. Interestingly CTNP’s did not exhibit any secondary complications against normal mice. The present findings give an experimental proof that the CTNP’s could serve as a promising candidate to overcome limitations of existing conventional cancer therapeutics.
Graphic abstract |
| doi_str_mv | 10.1007/s00775-019-01721-x |
| format | article |
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Clitoria ternatea
flower in the aqueous extract was investigated. Synthesized nanoparticles were characterized by using UV–Visible spectroscopy, followed by DLS, Zeta potential, XRD, FTIR, SEM, and AFM. The biocompatibility nature of CTNP’s was determined using erythrocytes model system. Cytotoxicity of CTNP’s against MCF-7 and EAC cells were determined by using MTT and Trypan blue exclusion method and their IC
50
was found to be 19.37 µg/mL and 24 µg/mL. Cytotoxic potential of CTNP’s was further confirmed by clonogenic assay. Further in vivo studies using EAC mice model supports the anti-cancer potential of silver nanoparticles. Results found that the CTNP’s effectively control the proliferation rate by inhibiting the ascites secretion and cellular density. Further quantification of VEGF, microvessel density counts and CAM assays show the anti-angiogenic potential of the CTNP’s. The apoptotic inducing activity of CTNP’s was confirmed by DNA fragmentation, fluorescent staining studies. More interestingly, EAC treated mice exhibit significant increase in lifespan (~ 2.25 fold) compared to control EAC mice. Interestingly CTNP’s did not exhibit any secondary complications against normal mice. The present findings give an experimental proof that the CTNP’s could serve as a promising candidate to overcome limitations of existing conventional cancer therapeutics.
Graphic abstract</description><identifier>ISSN: 0949-8257</identifier><identifier>EISSN: 1432-1327</identifier><identifier>DOI: 10.1007/s00775-019-01721-x</identifier><identifier>PMID: 31538255</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Angiogenesis ; Apoptosis ; Ascites ; Biochemistry ; Biocompatibility ; Biomedical and Life Sciences ; Clitoria ternatea ; Cytotoxicity ; DNA fragmentation ; Erythrocytes ; Inorganic chemistry ; Life Sciences ; Life span ; Microbiology ; Nanoparticles ; Original Paper ; Secretion ; Silver ; Spectroscopy ; Vascular endothelial growth factor ; Zeta potential</subject><ispartof>Journal of biological inorganic chemistry, 2019-10, Vol.24 (7), p.1115-1126</ispartof><rights>Society for Biological Inorganic Chemistry (SBIC) 2019</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-f4f635941f3ecae54f938dbf901797a4d8d9615687fc8aafc4af5046354075293</citedby><cites>FETCH-LOGICAL-c375t-f4f635941f3ecae54f938dbf901797a4d8d9615687fc8aafc4af5046354075293</cites><orcidid>0000-0003-4247-4944</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31538255$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Srinivas, Balaji Kyathegowdanadoddi</creatorcontrib><creatorcontrib>Shivamadhu, Madhu Chakkere</creatorcontrib><creatorcontrib>Siddappaji, Kiran Kumar</creatorcontrib><creatorcontrib>Krishnappa, Dharmappa Kattepura</creatorcontrib><creatorcontrib>Jayarama, Shankar</creatorcontrib><title>Angiosuppressive effects of bio-fabricated silver nanoparticles synthesis using Clitoria ternatea flower: an in vitro and in vivo approach</title><title>Journal of biological inorganic chemistry</title><addtitle>J Biol Inorg Chem</addtitle><addtitle>J Biol Inorg Chem</addtitle><description>Biosynthesis of silver nanoparticles (CTNP’s) by
Clitoria ternatea
flower in the aqueous extract was investigated. Synthesized nanoparticles were characterized by using UV–Visible spectroscopy, followed by DLS, Zeta potential, XRD, FTIR, SEM, and AFM. The biocompatibility nature of CTNP’s was determined using erythrocytes model system. Cytotoxicity of CTNP’s against MCF-7 and EAC cells were determined by using MTT and Trypan blue exclusion method and their IC
50
was found to be 19.37 µg/mL and 24 µg/mL. Cytotoxic potential of CTNP’s was further confirmed by clonogenic assay. Further in vivo studies using EAC mice model supports the anti-cancer potential of silver nanoparticles. Results found that the CTNP’s effectively control the proliferation rate by inhibiting the ascites secretion and cellular density. Further quantification of VEGF, microvessel density counts and CAM assays show the anti-angiogenic potential of the CTNP’s. The apoptotic inducing activity of CTNP’s was confirmed by DNA fragmentation, fluorescent staining studies. More interestingly, EAC treated mice exhibit significant increase in lifespan (~ 2.25 fold) compared to control EAC mice. Interestingly CTNP’s did not exhibit any secondary complications against normal mice. The present findings give an experimental proof that the CTNP’s could serve as a promising candidate to overcome limitations of existing conventional cancer therapeutics.
Graphic abstract</description><subject>Angiogenesis</subject><subject>Apoptosis</subject><subject>Ascites</subject><subject>Biochemistry</subject><subject>Biocompatibility</subject><subject>Biomedical and Life Sciences</subject><subject>Clitoria ternatea</subject><subject>Cytotoxicity</subject><subject>DNA fragmentation</subject><subject>Erythrocytes</subject><subject>Inorganic chemistry</subject><subject>Life Sciences</subject><subject>Life span</subject><subject>Microbiology</subject><subject>Nanoparticles</subject><subject>Original Paper</subject><subject>Secretion</subject><subject>Silver</subject><subject>Spectroscopy</subject><subject>Vascular endothelial growth factor</subject><subject>Zeta potential</subject><issn>0949-8257</issn><issn>1432-1327</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kU1v1DAQhi0EokvhD3BAlrhwCfXnJuZWrfiSKvUC58jrjLeusvbiSbbtX-BXMyUFJA492J6xn3dGnpex11K8l0K0Z0hbaxshHa1Wyeb2CVtJo1UjtWqfspVwxjWdsu0Je4F4LYTQVtrn7ERLq-nertjP87xLBefDoQJiOgKHGCFMyEvk21Sa6Lc1BT_BwDGNR6g8-1wOvk4pjIAc7_J0BZiQz5jyjm_GNJWaPJ-gZpJ5HsdyA_UD95mnzI9pqoXiYUmOFFPv4sPVS_Ys-hHh1cN5yr5_-vht86W5uPz8dXN-0QTd2qmJJq61dUZGDcGDNdHpbthGRzNwrTdDN7i1tOuujaHzPgbjoxWGNEa0Vjl9yt4tdantjxlw6vcJA4yjz1Bm7JVylnClLKFv_0Ovy0zfGonSNPy1kUoQpRYq1IJYIfaHmva-3vVS9PdO9YtTPTnV_3aqvyXRm4fS83YPw1_JH2sI0AuA9JR3UP_1fqTsLw6koUQ</recordid><startdate>20191001</startdate><enddate>20191001</enddate><creator>Srinivas, Balaji Kyathegowdanadoddi</creator><creator>Shivamadhu, Madhu Chakkere</creator><creator>Siddappaji, Kiran Kumar</creator><creator>Krishnappa, Dharmappa Kattepura</creator><creator>Jayarama, Shankar</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4247-4944</orcidid></search><sort><creationdate>20191001</creationdate><title>Angiosuppressive effects of bio-fabricated silver nanoparticles synthesis using Clitoria ternatea flower: an in vitro and in vivo approach</title><author>Srinivas, Balaji Kyathegowdanadoddi ; Shivamadhu, Madhu Chakkere ; Siddappaji, Kiran Kumar ; Krishnappa, Dharmappa Kattepura ; Jayarama, Shankar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-f4f635941f3ecae54f938dbf901797a4d8d9615687fc8aafc4af5046354075293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Angiogenesis</topic><topic>Apoptosis</topic><topic>Ascites</topic><topic>Biochemistry</topic><topic>Biocompatibility</topic><topic>Biomedical and Life Sciences</topic><topic>Clitoria ternatea</topic><topic>Cytotoxicity</topic><topic>DNA fragmentation</topic><topic>Erythrocytes</topic><topic>Inorganic chemistry</topic><topic>Life Sciences</topic><topic>Life span</topic><topic>Microbiology</topic><topic>Nanoparticles</topic><topic>Original Paper</topic><topic>Secretion</topic><topic>Silver</topic><topic>Spectroscopy</topic><topic>Vascular endothelial growth factor</topic><topic>Zeta potential</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Srinivas, Balaji Kyathegowdanadoddi</creatorcontrib><creatorcontrib>Shivamadhu, Madhu Chakkere</creatorcontrib><creatorcontrib>Siddappaji, Kiran Kumar</creatorcontrib><creatorcontrib>Krishnappa, Dharmappa Kattepura</creatorcontrib><creatorcontrib>Jayarama, Shankar</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biological inorganic chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Srinivas, Balaji Kyathegowdanadoddi</au><au>Shivamadhu, Madhu Chakkere</au><au>Siddappaji, Kiran Kumar</au><au>Krishnappa, Dharmappa Kattepura</au><au>Jayarama, Shankar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Angiosuppressive effects of bio-fabricated silver nanoparticles synthesis using Clitoria ternatea flower: an in vitro and in vivo approach</atitle><jtitle>Journal of biological inorganic chemistry</jtitle><stitle>J Biol Inorg Chem</stitle><addtitle>J Biol Inorg Chem</addtitle><date>2019-10-01</date><risdate>2019</risdate><volume>24</volume><issue>7</issue><spage>1115</spage><epage>1126</epage><pages>1115-1126</pages><issn>0949-8257</issn><eissn>1432-1327</eissn><abstract>Biosynthesis of silver nanoparticles (CTNP’s) by
Clitoria ternatea
flower in the aqueous extract was investigated. Synthesized nanoparticles were characterized by using UV–Visible spectroscopy, followed by DLS, Zeta potential, XRD, FTIR, SEM, and AFM. The biocompatibility nature of CTNP’s was determined using erythrocytes model system. Cytotoxicity of CTNP’s against MCF-7 and EAC cells were determined by using MTT and Trypan blue exclusion method and their IC
50
was found to be 19.37 µg/mL and 24 µg/mL. Cytotoxic potential of CTNP’s was further confirmed by clonogenic assay. Further in vivo studies using EAC mice model supports the anti-cancer potential of silver nanoparticles. Results found that the CTNP’s effectively control the proliferation rate by inhibiting the ascites secretion and cellular density. Further quantification of VEGF, microvessel density counts and CAM assays show the anti-angiogenic potential of the CTNP’s. The apoptotic inducing activity of CTNP’s was confirmed by DNA fragmentation, fluorescent staining studies. More interestingly, EAC treated mice exhibit significant increase in lifespan (~ 2.25 fold) compared to control EAC mice. Interestingly CTNP’s did not exhibit any secondary complications against normal mice. The present findings give an experimental proof that the CTNP’s could serve as a promising candidate to overcome limitations of existing conventional cancer therapeutics.
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| subjects | Angiogenesis Apoptosis Ascites Biochemistry Biocompatibility Biomedical and Life Sciences Clitoria ternatea Cytotoxicity DNA fragmentation Erythrocytes Inorganic chemistry Life Sciences Life span Microbiology Nanoparticles Original Paper Secretion Silver Spectroscopy Vascular endothelial growth factor Zeta potential |
| title | Angiosuppressive effects of bio-fabricated silver nanoparticles synthesis using Clitoria ternatea flower: an in vitro and in vivo approach |
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