Glycine receptor is differentially expressed in the rat retina at early stages of streptozotocin-induced diabetes

• Published in: Neuroscience letters 2019-11, Vol.712, p.134506-134506, Article 134506
• Main Authors: Morales-Calixto, Elizabeth, Velázquez-Flores, Miguel Ángel, Sánchez-Chávez, Gustavo, Ruiz Esparza-Garrido, Ruth, Salceda, Rocío
• Format: Article
• Language: English
• Subjects: Diabetes; Glycine; Glycine receptor; Retina; Streptozotocin
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2019.134506

•GlyR subunits expression was studied in the retina at early diabetes induction in the rat.•α1 mRNA and protein expression were not altered by the diabetic condition at early diabetes induction in the rat.•The α2 mRNA and protein expression were modestly reduced from very early stages of diabetes induction. Meanwhile, the α3 protein expression was significantly reduced at 45 days of diabetes.•mRNA and protein expression of the α4 subunit were remarkably decreased on day 7 of diabetes, remaining only 30% on day 45 of diabetes.•mRNA, but not protein of the β subunit, was considerably increased.•The changes in αGlyR subunits expression suggest a perturbation in inhibitory specific retinal circuits, suggesting a possible relation to vision alterations caused by diabetes. Diabetes mellitus is a metabolic disease that leads to several complications which include retinopathy. Neuronal abnormalities have been reported to appear before microvasculature alterations. We analyzed the expression levels of GlyR subunits in the retina at 7, 20, and 45 days after streptozotocin-induced diabetes to gain insight into the pathogenesis of diabetic retinopathy. We determined the mRNA and protein expression by qPCR and western blot, respectively. The mRNA and protein expression of the α1 subunit was not altered over the study period; however, they were slightly reduced in α2 yet statistically significant. While protein expression of α3 subunit was only reduced at 45 days diabetes. The mRNA and protein expression of the α4 subunit was remarkably decreased since day 7 of diabetes, remaining only ∼20% on day 45 of diabetes. Surprisingly, the mRNA of the β subunit was highly increased, while its protein levels were not changed. The decrease in GlyR α subunits expression in the retina from diabetic animals suggest a perturbation in the inhibitory glycine signaling pathway, which might be related to the visual alterations observed in diabetes.