The effects of gasotransmitters inhibition on biochemical and haematological parameters and oxidative stress in propofol-anaesthetized Wistar male rats

• Published in: Canadian journal of physiology and pharmacology 2019-11, Vol.97 (11), p.1073-1079
• Main Authors: Djuric, M, Nikolic Turnic, T, Kostic, S, Stankovic, S, Radonjic, K, Djuric, D, Zivkovic, V, Jakovljevic, V, Stevanovic, P
• Format: Article
• Language: English
• Subjects: Alkaline phosphatase; Amylases; Anesthetics - pharmacology; Animals; Arginine; Biomarkers - blood; Blood lipids; Calcium-binding protein; Carbon monoxide; Cholesterol; Enzymes; Ethylenediaminetetraacetic acid; Fibrin; Fibrinogen; Gasotransmitters - antagonists & inhibitors; Glutathione; Hematologic Tests; Hematology; Hemostasis - drug effects; Homocysteine - blood; Hydrogen peroxide; Hydrogen sulfide; Injection; Lipoproteins; Male; NG-Nitroarginine methyl ester; Nitric oxide; Nitric-oxide synthase; Oxidative stress; Oxidative Stress - drug effects; Oxides; Pharmacology; Phenols; Phosphatase; Phosphatases; Physiology; Platelets; Propofol; Propofol - pharmacology; Protoporphyrin; Protoporphyrin IX; Rats; Rats, Wistar; Superoxide; Superoxide dismutase; Troponin; Troponin T; Zinc; Zinc protoporphyrin IX
• ISSN: 0008-4212; 1205-7541
• DOI: 10.1139/cjpp-2019-0029

This study aimed to investigate the effects of propofol through evaluating its interaction with nitric oxide (NO), hydrogen sulfide (H S), and carbon monoxide (CO). Wistar male rats were divided in 4 groups: (1) bolus injection of propofol (1% 10 mg/mL, 100 mg/kg bw, i.p.); (2) -nitro-l-arginine methyl ester (L-NAME; NO synthase inhibitor, 60 mg/kg bw, i.p.) + bolus injection of propofol (1% 10 mg/mL, 100 mg/kg bw, i.p.); (3) DL-propargylglycine (DL-PAG; H S synthase inhibitor, 50 mg/kg bw, i.p.) + bolus injection of propofol (1% 10 mg/mL, 100 mg/kg bw, i.p.); (4) zinc protoporphyrin IX (ZnPPIX; CO synthase inhibitor, 50 μmol/kg bw, i.p.) + bolus injection of propofol (1% 10 mg/mL, 100 mg/kg bw, i.p.). Increased levels of albumins, low-density lipoproteins, alkaline phosphatase, amylase, high-sensitivity Troponin T, and fibrinogen were found in L-NAME + propofol group. Platelet crit, platelet count, total cholesterol, and high-density lipoproteins were elevated in ZnPPIX + propofol group. Hydrogen peroxide was increased in all groups treated with gasotransmitters inhibitors. Reduced glutathione was reduced in all groups, superoxide dismutase activity only in L-NAME + propofol. The effect of propofol on various biochemical, haematological, and oxidative stress markers may be at least in part mediated through interaction with 3 estimated gasotransmitters.