Evaluation of biofilm of Vibrio anguillarum for oral vaccination of Asian seabass, Lates calcarifer (BLOCH, 1790)

• Published in: Fish & shellfish immunology 2019-11, Vol.94, p.746-751
• Main Authors: Ram, Mohit Kumar, Naveen Kumar, B.T., Poojary, Sathish Rama, Abhiman, P.B., Patil, Prakash, Ramesh, K.S., Shankar, K.M.
• Format: Article
• Language: English
• Subjects: Administration, Oral; Animals; Bacterial Vaccines - administration & dosage; Bacterial Vaccines - pharmacology; Bass; Biofilm oral vaccine; Biofilms; Brackishwater fish; Fish Diseases - prevention & control; Hot Temperature; Lates calcarifer; Vaccination - veterinary; Vaccines, Inactivated - administration & dosage; Vaccines, Inactivated - pharmacology; Vibrio - immunology; Vibrio - physiology; Vibrio Infections - prevention & control; Vibrio Infections - veterinary; Vibriosis
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2019.09.053

The present study evaluated the biofilm (BF) of Vibrio anguillarum for oral vaccination of Asian seabass, Lates calcarifer. An 80-day experiment was carried out in circular fiber-reinforced plastic (FRP) tanks using free cell (FC) and BF of Vibrio anguillarum with triplicate in each. Heat-inactivated FC and BF cells at 107, 1010 and 1013 CFU/g fish/d were fed to fish for 20 days, agglutination antibody titer estimated at each 10 days interval up to 60-day post vaccination. As compared to FC and control there was a significant increase in agglutinating antibody titer in the biofilm vaccinated fishes. Among the 3 doses, BF at 1010 cfu/g fish/d was considered the ideal dose for vaccination. Relative percentage survival (RPS) was higher in biofilm vaccinated fish (85.4%) compared to that with free cells (27.0%). The study demonstrated the better performance of V. anguillarum biofilm oral vaccine compared that with free cell vaccine in L. calcarifer. The study further supports better performance of biofilm vaccine model with one more bacterial pathogen in a high carnivore fish. •Vaccinated feed prepared by incorporating heat -inactivated Vibrio anguillarum biofilm (BF) and free cell (FC) vaccines.•Asian seabass were orally vaccinated with heat-inactivated FC and BF cells at 107, 1010 and 1013 CFU/g fish/d for 20 days.•Agglutinating antibody titer was significantly increased in the biofilm vaccinated fishes.•LD50 dose of Vibrio anguillarum detremined to be 106 CFU/ g fish.•BF vaccinated groups at 1010cfu/g fish/d showed the highest relative percentage survival (RPS) compare to control and FC.