Association Between MTHFR Gene Common Variants, Serum Homocysteine, and Risk of Early-Onset Coronary Artery Disease: A Case–Control Study

The common variants of the methylenetetrahydrofolate reductase ( MTHFR ) gene are related to the activity of the MTHFR enzyme and the concentrations of blood homocysteine (Hcy). This study was designed to investigate the associations of MTHFR in Chinese populations with early-onset coronary artery d...

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Published in:Biochemical genetics 2020-04, Vol.58 (2), p.245-256
Main Authors: Zhang, Shao-Yan, Xuan, Chao, Zhang, Xue-Chun, Zhu, Jie, Yue, Kang, Zhao, Peng, He, Guo-Wei, Lun, Li-Min, Tian, Qing-Wu
Format: Article
Language:English
Subjects:
Age
Alleles
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cardiovascular disease
Confidence intervals
Coronary artery
Coronary artery disease
Coronary vessels
Disease control
Genotypes
Health risk assessment
Heart diseases
Heterozygotes
Homocysteine
Human Genetics
Medical Microbiology
Methylenetetrahydrofolate reductase
Original Article
Plasma
Population genetics
Reductases
Risk analysis
Risk factors
Serum levels
Zoology
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creator Zhang, Shao-Yan
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description The common variants of the methylenetetrahydrofolate reductase ( MTHFR ) gene are related to the activity of the MTHFR enzyme and the concentrations of blood homocysteine (Hcy). This study was designed to investigate the associations of MTHFR in Chinese populations with early-onset coronary artery disease (EOCAD). The two common variants of the MTHFR gene were genotyped in 875 EOCAD patients and 956 controls using PCR, followed by direct sequencing of the PCR product. Serum levels of Hcy were measured using an automatic biochemistry analyzer. A significant association between the MTHFR -677C/T variant and the risk of EOCAD was detected in CC versus TT (odds ratio (OR) 1.456, 95% confidence interval (CI) 1.120–1.892), dominant genetic model (OR 1.266, 95% CI 1.027–1.546), and recessive genetic model (OR 1.306, 95% CI 1.040–1.639). Hcy was most abundant in TT genotype (18.31 ± 7.22 μmol/L), least abundant in CC genotype (11.37 ± 5.23 μmol/L), and detectable at intermediate levels in heterozygotes (15.25 ± 6.58 μmol/L). Elevated serum Hcy levels were an independent risk factor for EOCAD (OR adjust 1.431, 95% CI 1.135–1.763). Our findings indicated that the T allele of -677C/T MTHFR variant predisposes to high levels of Hcy, and that the T allele is an important risk factor for EOCAD in the Chinese population.
doi_str_mv 10.1007/s10528-019-09937-x
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subjects Age
Alleles
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cardiovascular disease
Confidence intervals
Coronary artery
Coronary artery disease
Coronary vessels
Disease control
Genotypes
Health risk assessment
Heart diseases
Heterozygotes
Homocysteine
Human Genetics
Medical Microbiology
Methylenetetrahydrofolate reductase
Original Article
Plasma
Population genetics
Reductases
Risk analysis
Risk factors
Serum levels
Zoology
title Association Between MTHFR Gene Common Variants, Serum Homocysteine, and Risk of Early-Onset Coronary Artery Disease: A Case–Control Study
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