Nocardia panacis sp. nov., a novel actinomycete with antiphytopathogen activity isolated from the rhizosphere of Panax notoginseng

• Published in: Antonie van Leeuwenhoek 2020-02, Vol.113 (2), p.165-174
• Main Authors: Hu, Jia-Yun, Li, Lei, Peng, Guang, Li, Yi-Qing, Xu, Li-Hua, Guan, Hui-Lin, Zhao, Li-Xing, Miao, Cui-Ping
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Cardiolipin; Deoxyribonucleic acid; DNA; Fatty acids; Gene sequencing; Life Sciences; Lipids; Medical Microbiology; Menaquinones; Microbiology; New species; Nocardia; Original Paper; Panax notoginseng; Phosphatidylethanolamine; Phosphatidylinositol; Phylogenetics; Phylogeny; Plant Sciences; Rhizosphere; rRNA 16S; Soil Science & Conservation
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-019-01326-z

Strain YIM PH21724 T was isolated from the rhizosphere of Panax notoginseng . Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain exhibits close phylogenetic relatedness to Nocardia kroppenstedtii N1286 T (97.70%), Nocardia farcinica NCTC 11134 T (97.67%) and Nocardia puris DSM 44599 T (97.40%). The menaquinones were identified as MK-9 (H 4 ), MK-8 (H 4 , ω -cyclo) and MK-8 (H 4 ), and the major fatty acids (> 10%) were identified as C 16:0 , C 18:1 ω9 c and C 18:0 10-methyl. The polar lipids were found to be composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and an unidentified lipid. The G + C content of the genomic DNA was determined to be 67.01 mol%. The phenotypic, chemotaxonomic, phylogenetic and genomic results clearly show strain YIM PH21724 T should be classified in the genus Nocardia and represents a novel species, for which the name Nocardia panacis sp. nov. is proposed. The type strain is YIM PH21724 T (= DSM 105904 T  = KCTC 49030 T  = CCTCC AA 2017043 T ).