Heat stress-induced expression of Px-pdrg and Px-aspp2 in insecticide-resistant and -susceptible Plutella xylostella

• Published in: Bulletin of entomological research 2020-04, Vol.110 (2), p.177-184
• Main Authors: Wang, Yu, Wang, Jing Nan, Chen, Xue Zhun, Hu, Qi Xing, Liu, Qi Qing, Wu, Gang
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Amino acids; Animals; Apoptosis; Apoptosis Regulatory Proteins - genetics; Apoptosis Regulatory Proteins - metabolism; Cell cycle; Chlorpyrifos; Cloning; Complementary DNA; Deoxyribonucleic acid; DNA; DNA damage; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Gene expression; Genes; Genetic Fitness; Heat; Heat stress; Heat tolerance; Heat-Shock Response; High temperature; Insect Proteins - genetics; Insect Proteins - metabolism; Insecticide resistance; Insecticide Resistance - genetics; Insecticides; Insects; Mitochondria; Molecular weight; Moths - genetics; Moths - metabolism; Nucleotide sequence; p53 Protein; Pesticides; Plutella xylostella; Proteins; Pupae; Sequence Analysis, DNA; Thermal cycling; Transcription
• ISSN: 0007-4853; 1475-2670
• DOI: 10.1017/S0007485319000543

p53, DNA damage regulated gene (PDRG) and apoptosis-stimulating p53 protein 2 (ASPP2) are p53-related genes which can promote apoptosis. The full-length cDNA sequence of the Px-pdrg and Px-aspp2 genes were characterized and their mRNA expression dynamics under heat stress were studied in diamondback moth (DBM) Plutella xylostella collected from Fuzhou, China. The full-length cDNA of Px-pdrg and Px-aspp2 spans 721 and 4201 bp, containing 395 and 3216 bp of the open reading frame, which encode a putative protein comprising 130 and 1072 amino acids with a calculated molecular weight of 14.58 and 118.91 kDa, respectively. As compared to 25°C, both Px-pdrg and Px-aspp2 were upregulated in chlorpyrifos-resistant (Rc) and -susceptible (Sm) strains of DBM adults and pupae under heat stress. In addition, Rc DBM showed a significantly higher expression level of Px-pdrg and Px-aspp2 in contrast to Sm DBM. The results indicate that high temperature can significantly promote apoptosis process, especially in Rc-DBM. Significant fitness cost in Rc-DBM might be associated with drastically higher transcript abundance of Px-pdrg and Px-aspp2 under the heat stress.