Metabolomics Based on UHPLC-Orbitrap-MS and Global Natural Product Social Molecular Networking Reveals Effects of Time Scale and Environment of Storage on the Metabolites and Taste Quality of Raw Pu-erh Tea

Raw Pu-erh tea (RPT) needs ageing before drinking. However, the influence from environment and time of storage on chemical profile and flavor of RPT is unclear. In this study, the RPTs stored in wet-hot or dry-cold environment for 1–9 years were assessed using metabolomics based on UHPLC-Orbitrap-MS...

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Published in:Journal of agricultural and food chemistry 2019-10, Vol.67 (43), p.12084-12093
Main Authors: Xu, Shanshan, Wang, Jing-Jing, Wei, Yuming, Deng, Wei-Wei, Wan, Xiaochun, Bao, Guan-Hu, Xie, Zhongwen, Ling, Tie-Jun, Ning, Jingming
Format: Article
Language:English
Subjects:
Camellia sinensis - chemistry
Camellia sinensis - classification
Camellia sinensis - metabolism
China
Chromatography, High Pressure Liquid
Ecosystem
Electronic Nose
Environment
Fermentation
Food Storage
Mass Spectrometry
Metabolomics
Plant Leaves - chemistry
Plant Leaves - classification
Plant Leaves - metabolism
Taste
Tea - chemistry
Time Factors
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Summary:Raw Pu-erh tea (RPT) needs ageing before drinking. However, the influence from environment and time of storage on chemical profile and flavor of RPT is unclear. In this study, the RPTs stored in wet-hot or dry-cold environment for 1–9 years were assessed using metabolomics based on UHPLC-Orbitrap-MS and global natural product social (GNPS) feature-based molecular networking as well as electronic tongue measurement. The results exhibited that the chemical profiles of RPTs were similar at an early stage but started to differentiate from each other at the 5th and the 7th year in wet-hot and dry-cold environments. The discriminating features including N-ethyl-2-pyrrolidinone-substituted flavan-3-ols (flavoalkaloids), unsaturated fatty acids, lysophosphatidylcholines, flavan-3-ols, amino acids, and flavonol-O-glycosides among the three chemical profiles were discovered and analyzed by means of multivariate statistics, GNPS multilibraries matching, and SIRIUS calculation. The metabolomic data were consistent with the results obtained through electronic tongue measurement.
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.9b05314