Leukapheresis cell concentration adjustment required for a successful recovery of HSC after cryopreservation

• Published in: Cryobiology 2020-02, Vol.92, p.21-25
• Main Authors: Mohammed, Rebar N., Ahmad, Dereen, Khoshnaw, Najmaddin, Hassan, Dastan O., Abdulrahman, Hanar A., Vacca, Michele, Possenti, Marco, Faruq, Diana, Omer, Harem, Muhamad, Salah, Majolino, Ignazio, Othman, Dosti N.
• Format: Article
• Language: English
• Subjects: Apheresis; Autologous transplantation; Stem cell; Viability test
• ISSN: 0011-2240; 1090-2392
• DOI: 10.1016/j.cryobiol.2019.09.011

The recovering of an adequate number of hematopoietic stem cells after cryopreservation is considered pivotal for successful transplantation. Various factors could influence the recovery of HSC following processing and cryopreservation. Therefore, leukapheresis product from thirty patients was cryopreserved in 10% DMSO in cryopreservation bags for their autologous bone marrow transplantation, and 2 ml were cryopreserved in cryovials for post-thaw viability assessment by flow cytometry. The percentage of viable HSCs recovered post-cryopreservation in leukapheresis product was significantly influenced by the concentration of the total nucleated cells cryopreserved per volume. Patients receiving a higher rate of viable HSCs resulted in earlier engraftment of both neutrophils and platelets, so they have been discharged earlier from the hospital. Furthermore, Storage temperature and duration played a role in the recovery of these cells and for the support of the findings, age of the patient at the time of collection did not show any impact on the recovery of this HSC post-cryopreservation. In conclusion, various influencing factors must be taken into consideration during the cryopreservation of HSCs, especially for poor mobilizing patients with a low number of collected hematopoietic stem cells.