Human Germline Cell Development: from the Perspective of Single-Cell Sequencing

Germline cells are the beginning of new individuals in multicellular animals, including humans. Our understanding of these cell types is limited by the difficulty of analyzing the precious and heterogeneous germline tissue samples. The rapid development of single-cell sequencing technologies provide...

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Bibliographic Details
Published in:Molecular cell 2019-10, Vol.76 (2), p.320-328
Main Authors: Wen, Lu, Tang, Fuchou
Format: Article
Language:English
Subjects:
Blastocyst - physiology
Chromatin Assembly and Disassembly
DNA Methylation
Embryonic Development - genetics
Epigenesis, Genetic
Female
Fetal Stem Cells - physiology
Gene Expression Regulation, Developmental
Genotype
Humans
Male
Ovum - physiology
Phenotype
Sequence Analysis, DNA
Single-Cell Analysis - methods
Spermatogenesis - genetics
Spermatozoa - physiology
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Summary:Germline cells are the beginning of new individuals in multicellular animals, including humans. Our understanding of these cell types is limited by the difficulty of analyzing the precious and heterogeneous germline tissue samples. The rapid development of single-cell sequencing technologies provides a chance for comprehensive profiling of the omics dynamics of human germline development. In this review, we discuss progress in analyzing the development of human germline cells, including preimplantation and implantation embryos, fetal germ cells (FGCs), and adult spermatogenesis by single-cell transcriptome and epigenome sequencing technologies. Germline cells are the beginning of new individuals in multicellular animals, including humans. Our understanding of these cell types is limited by the difficulty of analyzing the precious and heterogeneous germline tissue samples. The rapid development of single-cell sequencing technologies provides a chance for comprehensive profiling of the omics dynamics of human germline development. In this review, we discuss progress in analyzing the development of human germline cells, including preimplantation and implantation embryos, fetal germ cells (FGCs), and adult spermatogenesis by single-cell transcriptome and epigenome sequencing technologies.
ISSN:1097-2765
1097-4164
DOI:10.1016/j.molcel.2019.08.025