Quality assessment of cryopreserved biospecimens reveals presence of intact biomolecules

Recapitulation of tumor features in isolated biomolecules is preeminently dependent on obtaining reliable quality biospecimen. Moreover, quality assessment of biobanked specimens at regular intervals is an essential intervention for carrying out effective translational and clinical research. In the...

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Bibliographic Details
Published in:Journal of biophotonics 2019-12, Vol.12 (12), p.e201960048-n/a
Main Authors: Pansare, Kshama, Pillai, Divya, Parab, Saili, Singh, Saurav R., Kannan, Sadhana, Ludbe, Madan, Hole, Arti, Murali Krishna, C., Gera, Poonam
Format: Article
Language:English
Subjects:
biobank
Biomolecules
biospecimen
Breast
Cryopreservation
Deoxyribonucleic acid
DNA
Electrophoresis
Fluorescence
Fluorescence spectroscopy
Gel electrophoresis
Qualitative analysis
Quality
Quality assessment
Quality control
Raman spectra
Raman spectroscopy
Spectrum analysis
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Summary:Recapitulation of tumor features in isolated biomolecules is preeminently dependent on obtaining reliable quality biospecimen. Moreover, quality assessment of biobanked specimens at regular intervals is an essential intervention for carrying out effective translational and clinical research. In the current study, genomic DNA was extracted from 140 fresh frozen tissues of oral, breast and colorectal specimens cryopreserved over a period of 3 to 8 months (short term) and 3 to 4 years (long term). Quantification of genomic DNA by absorption and fluorescence spectroscopy confirmed high concentration while qualitative analysis by gel electrophoresis showed intact bands for 94% and 87% of short‐ and long‐term cohorts, respectively. PC‐LDA based classification of Raman spectra showed overlapping groups of both cohorts suggesting the quality of DNA being preserved irrespective of storage period. To the best of our knowledge this is the first Indian biobank study reporting quality analysis of biospecimens cryopreserved at different time periods. Quality assessment of tumour biobank specimens
ISSN:1864-063X
1864-0648
DOI:10.1002/jbio.201960048