Effects of bazedoxifene and low-intensity aerobic exercise on bone and fat parameters in ovariectomized rats

• Published in: Journal of bone and mineral metabolism 2020-03, Vol.38 (2), p.179-187
• Main Authors: Yuasa, Yusuke, Miyakoshi, Naohisa, Kasukawa, Yuji, Nagahata, Itsuki, Akagawa, Manabu, Ono, Yuichi, Sato, Chiaki, Tsuchie, Hiroyuki, Nozaka, Koji, Nagasawa, Hiroyuki, Hongo, Michio, Shimada, Yoichi
• Format: Article
• Language: English
• Subjects: Adipocytes; Body fat; Bone marrow; Bone mass; Bone mineral density; Dual energy X-ray absorptiometry; Dyslipidemia; Exercise; Femur; Fitness equipment; Mechanical properties; Medicine; Medicine & Public Health; Metabolic Diseases; Original Article; Orthopedics; Osteoporosis; Ovariectomy; Post-menopause; Selective estrogen receptor modulators; Spine (lumbar); Tibia
• ISSN: 0914-8779; 1435-5604
• DOI: 10.1007/s00774-019-01045-5

Introduction Postmenopausal osteoporosis and dyslipidemia are well-known skeletal and metabolic changes in middle-aged women. We investigated the effects of combined treatments with a selective estrogen receptor modulator (SERM) and exercise on bone and fat parameters in ovariectomized (OVX) rats. Materials and Methods Sixteen-week-old female Sprague–Dawley rats underwent bilateral ovariectomy, and rats were randomized to BZA (bazedoxifene at 0.3 mg/kg/day), Exe (treadmill exercise at 12–15 m/min, 60 min/day, 5 days/week), Comb (BZA and Exe), and Cont (control treated with vehicle and no exercise) groups 8 weeks after ovariectomy. After 4 or 8 weeks of treatment, bone mineral density (BMD) of the total femur and lumbar spine and whole-body percentage fat mass were determined by dual-energy X-ray absorptiometry, and mechanical testing of the femoral shaft, and bone and fat histomorphometric analyses of the proximal tibia were performed. Results Treadmill exercise had decreased bone marrow adipocytes from 4 weeks of treatment and whole-body percentage fat mass at 8 weeks. BZA increased BMD at the lumbar spine and decreased the whole-body percentage fat mass from 4 weeks and bone marrow adipocytes at 8 weeks. Combination therapy increased BMD for the lumbar spine and decreased bone marrow adipocytes and whole-body percentage fat mass from 4 weeks. Conclusion Combination therapy with BZA and exercise appears effective to improve bone and fat parameters in OVX rats.