Highly efficient and selective antimicrobial isonicotinylhydrazide-coated polyoxometalate-functionalized silver nanoparticles

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2019-12, Vol.184, p.110522-110522, Article 110522
• Main Authors: Umapathi, Akhela, Nagaraju, Navya P., Madhyastha, Harishkumar, Jain, Devendra, Srinivas, Sangly P., Rotello, Vincent M., Daima, Hemant Kumar
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - chemical synthesis; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antimicrobial; Cytotoxicity; Isoniazid - chemistry; Isoniazid - pharmacology; Isonicotinylhydrazide; Metal Nanoparticles - chemistry; Mice; Microbial Sensitivity Tests; Molecular Structure; Nanozyme; Particle Size; Polyoxometalates; Silver - chemistry; Silver - pharmacology; Silver nanoparticles; Staphylococcus aureus - drug effects; Surface Properties; Tungsten Compounds - chemistry; Tungsten Compounds - pharmacology
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2019.110522

Highlights •A sequential approach to prepare isonicotinylhydrazide-coated polyoxometalates functionalized Ag nanoparticles is presented.•Isonicotinylhydrazide and polyoxometalates developed a surface corona and imparted biological potential on nanoparticles.•Enhanced in-vitro nanozyme activity, high antimicrobial potential, and controlled cytotoxicity on fibroblasts is reported. [Display omitted] With the rapidly approaching post-antibiotic era, new and effective combinations of antibiotics are imperative to combat multiple drug resistance (MDR). We have synthesized multimodal antimicrobials that integrate the antibiotic isonicotinylhydrazide (INH), silver nanoparticles (AgNPsINH), and two different polyoxometalates (POMs) namely, phosphotungstic acid (PTA) and phosphomolybdic acid (PMA) to prepare AgNPsINH@PTA and AgNPsINH@PMA, respectively. AgNPsINH have peroxidase-like (nanozyme) activity and very high antibacterial potential toward S. aureus, which was further enhanced upon modification with POMs. The selectivity of these functional nanozymes was evaluated with m5S mouse fibroblasts using WST-8, LDH viability, in vitro reactive oxygen species (ROS) generation assays, and crystal violet morphological studies. These investigations showed very low cytotoxicity for the nanoparticles compared to free metal ions (Ag+), pristine POMs and INH, demonstrating the ability of multifunctional materials to provide efficient and selective antimicrobials.