Inhibition of Rhizoctonia solani RhCh-14 and Pythium ultimum PyFr-14 by Paenibacillus polymyxa NMA1017 and Burkholderia cenocepacia CACua-24: A proposal for biocontrol of phytopathogenic fungi

• Published in: Microbiological research 2020-01, Vol.230, p.126347-126347, Article 126347
• Main Authors: Chávez-Ramírez, Belén, Kerber-Díaz, Jeniffer Chris, Acoltzi-Conde, Marí Carmen, Ibarra, J. Antonio, Vásquez-Murrieta, María-Soledad, Estrada-de los Santos, Paulina
• Format: Article
• Language: English
• Subjects: Antifungal Agents - metabolism; Antifungal Agents - pharmacology; Biological control; Burkholderia cenocepacia; Burkholderia cenocepacia - chemistry; Burkholderia cenocepacia - metabolism; Nicotiana - growth & development; Nicotiana - microbiology; Paenibacillus polymyxa; Paenibacillus polymyxa - chemistry; Paenibacillus polymyxa - metabolism; Pest Control, Biological - methods; Plant Diseases - microbiology; Pythium - drug effects; Pythium - growth & development; Pythium - physiology; Pythium ultimum; Rhizoctonia - drug effects; Rhizoctonia - growth & development; Rhizoctonia - physiology; Rhizoctonia solani
• ISSN: 0944-5013; 1618-0623
• DOI: 10.1016/j.micres.2019.126347

Biocontrol has emerged in recent years as an alternative to pesticides. Given the importance of environmental preservation using biocontrol, in this study two antagonistic bacteria against phytopathogenic fungi were isolated and evaluated. These bacterial strains, identified as Paenibacillus polymyxa NMA1017 and Burkholderia cenocepacia CACua-24, inhibited (70 to 80%) the development of two phytopathogens of economic importance: the fungus Rhizoctonia solani RhCh-14, isolated from chili pepper, and the oomycete Pythium ultimum PyFr-14, isolated from tomato. The spectrum was not limited to the previous pathogens, but also to other phytopathogenic fungus, some bacteria and other oomycetes. Fungi-bacteria microcultures observed with optical and scanning electron microscopy revealed hyphae disintegration and pores formation. The antifungal activity was found also in the supernatant, suggesting a diffusible compound is present. Innocuous tests on tobacco leaves, blood agar, bean seed germination and in Galleria mellonella larvae showed that strain NMA1017 has the potential to be a biocontrol agent. Greenhouse experiments with bean plants inoculated with P. polymyxa exhibited the efficacy to inhibit the growth of R. solani and P. ultimum. Furthermore, P. polymyxa NMA1017 showed plant growth promotion activities, such as siderophore synthesis and nitrogen fixation which can contribute to the crop development.