Total flavones of Rhododendron simsii Planch flower protect rat hippocampal neuron from hypoxia-reoxygenation injury via activation of BKCa channel

Abstract Objectives To study the effects of total flavones of Rhododendra simsii Planch flower (TFR) on hypoxia/reoxygenation (H/R) injury in rat hippocampal neurons and its underlying mechanism. Method Model of H/R was established in newborn rat primary cultured hippocampal neuron. Lactate dehydrog...

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Published in:Journal of pharmacy and pharmacology 2020-01, Vol.72 (1), p.111-120
Main Authors: Guo, Yan, Yu, Xiao-Meng, Chen, Shuo, Wen, Ji-Yue, Chen, Zhi-Wu
Format: Article
Language:English
Subjects:
Activation
Apoptosis
BKCachannel
Cell viability
Flavones
hippocampal neuron
Hippocampus
Hypoxia
hypoxia-reoxygenation injury
L-Lactate dehydrogenase
Lactic acid
Malondialdehyde
Neurons
Neuroprotection
Phosphopyruvate hydratase
Protein expression
TFR
Viability
Western blotting
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Summary:Abstract Objectives To study the effects of total flavones of Rhododendra simsii Planch flower (TFR) on hypoxia/reoxygenation (H/R) injury in rat hippocampal neurons and its underlying mechanism. Method Model of H/R was established in newborn rat primary cultured hippocampal neuron. Lactate dehydrogenase (LDH) and neuron-specific enolase (NSE) activity as well as malondialdehyde (MDA) content in cultured supernatants of the neurons were examined. Methyl thiazolyl tetrazolium assay and Hoechst33258 staining were, respectively, used to detect cell viability and apoptosis of neurons. Protein expression and current of BKCa channel were assessed by using Western blotting and whole-cell patch-clamp methods, respectively. Key findings In the ranges of 3.7–300 mg/l, TFR significantly inhibited H/R-induced decrease of neuronal viability and increases of LDH, NSE and MDA in the supernatants as well as apoptosis; TFR 33.3, 100 and 300 mg/l markedly increased current of BKCa channel rather than the BKCa channel protein expression in the neurons. Conclusions Total flavones of R. simsii Planch flower had a protective effect against H/R injury in rat hippocampal neuron, and activation of BKCa channel may contribute to the neuroprotection.
ISSN:0022-3573
2042-7158
DOI:10.1111/jphp.13178