Protective effects of Croton hookeri on streptozotocin-induced diabetic nephropathy

• Published in: Food and chemical toxicology 2020-01, Vol.135, p.110873-110873, Article 110873
• Main Authors: Kundu, Amit, Dey, Prasanta, Sarkar, Pradipta, Karmakar, Sanmoy, Tae, In Hwan, Kim, Kyeong Seok, Park, Jae Hyeon, Lee, Su Hyun, Lee, Byung Mu, Renthlei, Lalmuanawmi, Puia, Zothan, Kim, Hyung Sik
• Format: Article
• Language: English
• Subjects: Animals; Biomarkers - urine; Blood Glucose - metabolism; Body Weight - drug effects; Croton - chemistry; Croton hookeri; Cytokines - metabolism; Diabetic Nephropathies - metabolism; Diabetic Nephropathies - prevention & control; Diabetic Nephropathies - urine; Diabetic nephropathy; Functional Food; Glycation End Products, Advanced - metabolism; Inflammation; Inflammation Mediators - metabolism; Kidney - drug effects; Kidney - metabolism; Kidney - physiopathology; Male; Organ Size - drug effects; Oxidative stress; Oxidative Stress - drug effects; Plant Extracts - pharmacology; Rats; Rats, Sprague-Dawley; Streptozocin; Urinary biomarkers
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2019.110873

In this study, the protective effects of Croton hookeri (CH) extract on renal injury were investigated in streptozotocin (STZ)-induced diabetic rats. Diabetes was induced by a single injection of STZ (45 mg/kg) to Sprague-Dawley rats. After 5 days, CH extract (200 mg/kg) was administered daily by oral gavage for 2 weeks. Administration of CH extracts significantly reduced blood glucose levels in STZ-induced diabetic rats. STZ-induced changes in total cholesterol, LDL, HDL, ALT, AST, BUN, and serum creatinine levels were significantly restored by treatment with CH extract. Abnormal levels of SOD, catalase, glutathione, and oxidized GSH (GSSG) in STZ-treated rats were also significantly recovered by CH extract treatment. CH extract markedly reduced the expression of collagen-1, fibronectin, and α-SMA in the kidney of STZ-induced diabetic rats. In particular, oxidative DNA damages, MDA, TGF-β, IL-1β, and IL-6 levels were significantly reduced in STZ-treated rats following treatment with CH extract, whereas IL-10 showed opposite trend. STZ-induced SIRT1, SIRT3 downregulation and cloudin-1 upregulation in the kidney were dramatically recovered by CH extract treatment. Our data suggest that CH extract protects against diabetic-induced nephropathy by inhibiting oxidative stress and inflammation. Therefore, it has potential as a food supplement to alleviate renal dysfunction caused by diabetes-induced nephropathy. [Display omitted] •CH extracts protects streptozocin-induced diabetic nephropathy.•CH extracts reduces urinary excretion of kidney injury biomarkers.•CH extracts protects streptozocin-induced oxidative stress in the kidney.•CH extracts protects streptozocin-induced inflammation in the kidney.