The role of N6‐methyladenosine modification on diapause in silkworm (Bombyx mori) strains that exhibit different voltinism

N6‐methyladenosine (m6A) plays a key role in regulating gene expression in myriad organisms. Diapause is an important plastic phenotype that allows insects to survive under specific environmental conditions. However, the diapause molecular mechanism remains unknown. In this study, we analyzed the ph...

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Bibliographic Details
Published in:Molecular reproduction and development 2019-12, Vol.86 (12), p.1981-1992
Main Authors: Jiang, Tao, Li, Jiashuang, Qian, Ping, Xue, Peng, Xu, Jin, Chen, Yanrong, Zhu, Juan, Tang, Shunming, Zhao, Qiaoling, Qian, Heying, Shen, Xingjia
Format: Article
Language:English
Subjects:
Bombyx mori
Diapause
Eggs
Environmental conditions
Epigenetics
Gene expression
Gene regulation
Hydrochloric acid
N6-methyladenosine
Phenotypes
Phenotypic plasticity
Phylogeny
response
RNA methylation
Strains (organisms)
Voltinism
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Summary:N6‐methyladenosine (m6A) plays a key role in regulating gene expression in myriad organisms. Diapause is an important plastic phenotype that allows insects to survive under specific environmental conditions. However, the diapause molecular mechanism remains unknown. In this study, we analyzed the phylogenetics of genes related to the m6A modification complex in the silkworm (Bombyx mori) based on identified sequences from other organisms. We detected the expression of these genes during different developmental phases from four strains with different voltinism. We also determined total m6A content in cells treated with different diapause hormone concentrations or eggs exposed to hydrochloric acid. Our data revealed that m6A‐modification‐related gene expression and m6A content were greater in diapause‐destinated compared to nondiapause‐destined strains. Our findings suggest that m6A modification may provide significant epigenetic regulation of diapause‐related genes in the silkworm. N6‐methyladenosine (m6A)‐modification‐related gene expression and m6A content were greater in diapause‐destinated compared to nondiapause‐destined strains. Our findings suggest that m6A modification may provide significant epigenetic regulation of diapause‐related genes in the silkworm.
ISSN:1040-452X
1098-2795
DOI:10.1002/mrd.23283