Development of a disposable micro-capillary film grafted with peptide ligands for immunoadsorption

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2019-10, Vol.1129, p.121801-121801, Article 121801
• Main Authors: Li, Jiawen, Lin, Dongqiang, Yao, Shanjing, Slater, N.K.H., Zhang, Qilei
• Format: Article
• Language: English
• Subjects: Adsorption; Autoimmune diseases; Chromatography, Affinity - methods; Humans; Immunoglobulin; Immunoglobulin G - isolation & purification; Kinetics; Ligands; Microporous micro-capillary film; Molecular Structure; Peptide ligands; Peptides - chemistry; Porosity; Serum Albumin, Human - chemistry; Structure-Activity Relationship; Surface Properties; Vinyl Compounds - chemistry
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2019.121801

Traditional chromatographic techniques used in downstream processes of biomolecule manufacturing are often time-consuming and expensive. In this study, a cost-effective microporous micro-capillary film (MMCF) composed of ethylenevinyl alcohol (EVOH) was evaluated for its potential application in immunoadsorption with high process efficiency. A peptide ligand Ac-Phe-Tyr-His-Glu (Ac-FYHE) was immobilized on the inner surface of MMCF for selective binding of human immunoglobulin (hIgG). The porous structure and chemical properties of the prepared MMCF were confirmed by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). hIgG (2 mg/ml) adsorption studies demonstrated that the binding process followed a Langmuir isotherm with equilibrium adsorption capacities of 9.31 and 3.47 mg/ml adsorbent under static and dynamic conditions, respectively. Moreover, the membrane showed good flowrate tolerance when studied under flowrates of 0.5 ml/min to 10 ml/min. hIgG purity was 88.2% when obtained from an hIgG (2 mg/ml) and HSA (8 mg/ml) mixture and the purity remained over 80.0% when hIgG concentrations increased in the mixtures. Moreover, purity of 82.3% was achieved when removing hIgG directly from human serum. The MMCF-Ac-FYHE affinity column is expected to selectively remove hIgG from blood for the treatment of autoimmune diseases with high efficiency and cost effectiveness. •A microporous micro-capillary membrane for potential immunoadsorption was developed.•The prepared membrane column can selectively bind hIgG with high flowrate tolerance.•The prepared membrane column was cost-effective for disposable applications.