Identification of ribosomal sites and karyotype analysis in Festuca ulochaeta Steud. and Festuca fimbriata Ness., grasses native to Brazil

Festuca L. has more than 600 perennial species described, which makes it the largest genus within the family Poaceae. In Brazil, only two native species of Festuca have been described, for which cytogenetic studies need to be strengthened: Festuca ulochaeta and Festuca fimbriata . The aim of this st...

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Bibliographic Details
Published in:Molecular biology reports 2020-01, Vol.47 (1), p.261-267
Main Authors: Ferreira, Marco Túlio Mendes, Chaves, Ana Luisa Arantes, Rocha, Laiane Corsini, da Silva, Leonardo Nogueira, Kalthuk-Santos, Eliane, Techio, Vania Helena
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Biomedical and Life Sciences
Brazil
Chromosomes
Cytogenetics
Diploidy
DNA, Ribosomal - genetics
DNA, Ribosomal - metabolism
Festuca
Festuca - genetics
Festuca - metabolism
Gene mapping
Histology
In Situ Hybridization, Fluorescence - methods
Indigenous species
Karyotype
Karyotypes
Life Sciences
Morphology
Original Article
Poaceae - genetics
Poaceae - metabolism
Ribosomes - genetics
RNA, Ribosomal, 5S - genetics
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Summary:Festuca L. has more than 600 perennial species described, which makes it the largest genus within the family Poaceae. In Brazil, only two native species of Festuca have been described, for which cytogenetic studies need to be strengthened: Festuca ulochaeta and Festuca fimbriata . The aim of this study was to characterize the karyotypes of F. ulochaeta and F. fimbriata based on the mapping of rDNA sites. The FISH was performed with 35S and 5S rDNA probes. Both species have 42 chromosomes, of which 36 were metacentric and six were submetacentric. Festuca fimbriata has two pairs of 35S rDNA sites, one located on the metacentric pair 4, in an interstitial position, and one at the submetacentric pair 14 in the proximal position. Festuca ulochaeta has one pair of 35S rDNA in interstitial-proximal position in the metacentric pair 3. Both species showed 5S rDNA sites only on chromosome pair 21 in the terminal position of the short arm. The analysis of the chromosomal characteristics indicates that these species have a symmetrical karyotype and allopolyploid origin.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-019-05126-6