Development of a double‐antibody sandwich ELISA for sensitive detection of Yersinia pestis

We developed a biotin–streptavidin‐based sandwich ELISA for the sensitive and specific detection of Yersinia pestis. In this assay, the F1 capsular protein and Y. pestis were captured by anti‐F1 mouse monoclonal antibody followed by detection with biotinylated‐anti‐F1 rabbit polyclonal antibody and...

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Bibliographic Details
Published in:Microbiology and immunology 2020-01, Vol.64 (1), p.72-75
Main Authors: Choi, Sang‐Yoon, Rhie, Gi‐eun, Jeon, Jun Ho
Format: Article
Language:English
Subjects:
Animals
Antibodies, Bacterial - immunology
Antibodies, Monoclonal - immunology
Antigens, Bacterial - immunology
Bacterial Proteins - genetics
Bacterial Proteins - immunology
Biotin
detection
Diagnostic Tests, Routine - methods
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
F1 capsule
Humans
Limit of Detection
Monoclonal antibodies
Rabbits
sandwich ELISA, Yersinia pestis
Sensitivity and Specificity
Streptavidin
Yersinia pestis
Yersinia pestis - genetics
Yersinia pestis - immunology
Yersinia pestis - isolation & purification
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Summary:We developed a biotin–streptavidin‐based sandwich ELISA for the sensitive and specific detection of Yersinia pestis. In this assay, the F1 capsular protein and Y. pestis were captured by anti‐F1 mouse monoclonal antibody followed by detection with biotinylated‐anti‐F1 rabbit polyclonal antibody and HRP‐conjugated streptavidin. The developed F1 ELISA could detect not only the F1 protein up to 29 and 17 pg/ml but also Y. pestis up to 177.8 and 129.2 CFU/ml in PBS buffer and human serum, respectively. In addition, the F1 ELISA did not show any cross‐reactivity with various proteins and bacterial strains.
ISSN:0385-5600
1348-0421
DOI:10.1111/1348-0421.12751