miR‐298 plays a pivotal role in colon cancer invasiveness by targeting PTEN

Evidence indicate that the miR‐298 dysregulation might associate with colorectal cancer (CRC) development. Herein, we evaluated the effect of miR‐298 dysregulation on colon cancer invasiveness. First, metabolic activity, cell cycle progression, apoptosis, and invasion of miR‐298 overexpressed/knocke...

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Bibliographic Details
Published in:Journal of cellular physiology 2020-05, Vol.235 (5), p.4335-4350
Main Authors: Arabsorkhi, Zahra, Gharib, Ehsan, Yaghmoorian Khojini, Javad, Farhadieh, Mohammad‐Erfan, Nazemalhosseini‐Mojarad, Ehsan, Zali, Mohammad Reza
Format: Article
Language:English
Subjects:
Adult
Aged
Apoptosis
Cell Cycle
Cell Line, Tumor
Cell proliferation
Cell Survival
Colon
Colon cancer
Colonic Neoplasms - metabolism
Colonic Neoplasms - pathology
Colorectal cancer
Colorectal carcinoma
Comparative analysis
Confidence intervals
Correlation analysis
Evaluation
Female
Gene expression
Gene Expression Regulation, Neoplastic - physiology
Homology
Humans
Intracellular signalling
invasion
Invasiveness
Lymph nodes
Male
Metastases
Metastasis
MicroRNAs - genetics
MicroRNAs - metabolism
microRNA‐298
Middle Aged
Multivariate analysis
Neoplasm Invasiveness - genetics
Plasmas (physics)
PTEN
PTEN Phosphohydrolase - genetics
PTEN Phosphohydrolase - metabolism
PTEN protein
Regression analysis
Sequence Analysis, RNA
Statistical analysis
Survival
Tensin
Therapeutic applications
Tumors
Up-Regulation
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Summary:Evidence indicate that the miR‐298 dysregulation might associate with colorectal cancer (CRC) development. Herein, we evaluated the effect of miR‐298 dysregulation on colon cancer invasiveness. First, metabolic activity, cell cycle progression, apoptosis, and invasion of miR‐298 overexpressed/knocked out colon cancer cells were examined and combined with their transcriptome analysis data for better visualization of miR‐298 intracellular signaling networks. Interaction between miR‐298 and its target was evaluated with luciferase assay and validated using western blot analysis. The proportion of abnormal miR‐298 level was investigated in tumor samples, matched normal adjacent tissues, and plasmas of 100 CRC patients, and also compared with 100 normal plasma samples. The Mann–Whitney U test was performed to assess miR‐298 differences among the studied groups, and the correlation between miR‐298 and the risk of CRC was shown by univariate and multivariate logistic regression. The data indicate that miR‐298 overexpression promoted proliferation and metastasis in CRC cells via targeting phosphatase and tensin homolog. Comparative analysis of CRC tumors, normal adjacent tissues, and plasmas indicated a significant miR‐298 upregulation in tumors and plasmas (1.72‐fold and 1.65‐fold, respectively; p 
ISSN:0021-9541
1097-4652
DOI:10.1002/jcp.29310