Combined use of ivermectin, dimethyl sulfoxide, mineral oil and nematophagous fungi to control Rhabditis spp

• Published in: Veterinary parasitology 2019-11, Vol.275, p.108924-108924, Article 108924
• Main Authors: Ferraz, Carolina Magri, Sobral, Samilla Alves, Senna, Caio Colodette, Junior, Otavio Fidelis, Moreira, Tiago Facury, Tobias, Fernando Luiz, de Freitas Soares, Filippe Elias, Geniêr, Hugo Leonardo André, Vilela, Vinicius Longo Ribeiro, Lima, José Antônio Correia, de Araújo, Jackson Victor, Braga, Fabio Ribeiro
• Format: Article
• Language: English
• Subjects: Animals; Antiparasitic Agents - pharmacology; Antiparasitic Agents - therapeutic use; Ascomycota - physiology; Biological control; Cattle; Cattle Diseases - drug therapy; Cattle Diseases - parasitology; Cattle Diseases - prevention & control; Dairying; Dimethyl Sulfoxide - pharmacology; Dimethyl Sulfoxide - therapeutic use; Duddingtonia - physiology; Female; Ivermectin - pharmacology; Ivermectin - therapeutic use; Male; Mineral Oil - pharmacology; Mineral Oil - therapeutic use; Mitosporic Fungi - physiology; Nematophagous fungi; Otitis Externa - drug therapy; Otitis Externa - parasitology; Otitis Externa - prevention & control; Otitis Externa - veterinary; Parasitic otitis; Rhabditida Infections - drug therapy; Rhabditida Infections - microbiology; Rhabditida Infections - prevention & control; Rhabditida Infections - veterinary; Rhabditoidea - drug effects; Rhabditoidea - microbiology
• ISSN: 0304-4017; 1873-2550
• DOI: 10.1016/j.vetpar.2019.108924

•The use of dimethyl sulfoxide 1% and mineral oil 100% in with conidia fungi was eficiente.•The combination of AC001 or NF34 + dimethyl sulfoxide 1% reduced the mean quantity of nematodes in 98.5%. Rhabditis spp., is a nematode known to cause otitis externa, an infection difficult to control, in cattle reared within tropical regions. The objective of this study was to assess the combined use of ivermectin 1%, dimethyl sulfoxide 1% and mineral oil 100% containing nematophagous fungi of both Duddingtonia flagrans (AC001) and Monacrosporium thaumasium (NF34) species to control in vitro Rhabditis spp. Thus, 12 experimental groups were designed with eight replicates each: G1 (nematodes + AC001); G2 (nematodes + NF34); G3 (nematodes + ivermectin 1%/positive control); G4 (nematodes + dimethyl sulfoxide 1%/positive control); G5 (nematodes + mineral oil 100%/positive control); G6 (nematodes + AC001 + ivermectin 1%); G7 (nematodes + NF34 + ivermectin 1%); G8 (nematodes + AC001 + mineral oil 100%); G9 (nematodes + NF34 + mineral oil 100%); G10 (nematodes + AC001 + dimethyl sulfoxide 1%); G11 (nematode + NF34 + dimethyl sulfoxide 1%); G12 (nematode + distilled water/negative control). The results demonstrated that all experimentally treated groups differed statistically (p