A statistical model for activation of Factor C by binding to LPS aggregates

Published data on Factor C activity at various LPS and Lipid A concentrations (Nakamura et al. in Eur J Biochem 176:89, 1988; Kobayashi et al. in J Biol Chem 37:25987, 2014) were rearranged to show that Factor C exhibited its maximum activity at a specific concentration of LPS. A statistical model w...

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Bibliographic Details
Published in:European biophysics journal 2019-12, Vol.48 (8), p.743-747
Main Authors: Miyagawa, Y., Kikuchi, K., Tsuchiya, M., Adachi, S.
Format: Article
Language:English
Subjects:
Activation
Aggregates
Arthropod Proteins - chemistry
Arthropod Proteins - metabolism
Binding
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Cell Biology
Dimerization
Dimers
Enzyme Precursors - chemistry
Enzyme Precursors - metabolism
Life Sciences
Lipid A
Lipid A - metabolism
Lipid A - pharmacology
Lipids
Lipopolysaccharides - chemistry
Lipopolysaccharides - metabolism
Lipopolysaccharides - pharmacology
Mathematical models
Membrane Biology
Models, Molecular
Molecular interactions
Nanotechnology
Neurobiology
Original Article
Protein Binding
Protein Multimerization - drug effects
Protein Structure, Quaternary
Serine Endopeptidases - chemistry
Serine Endopeptidases - metabolism
Statistical models
Citations: Items that this one cites
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Summary:Published data on Factor C activity at various LPS and Lipid A concentrations (Nakamura et al. in Eur J Biochem 176:89, 1988; Kobayashi et al. in J Biol Chem 37:25987, 2014) were rearranged to show that Factor C exhibited its maximum activity at a specific concentration of LPS. A statistical model was proposed for examining whether a single LPS molecule binding activates Factor C (monomeric activation) or dimerization of Factor C is necessary for the activation (dimeric activation). In the monomeric activation model the plots of the relative activity of Factor C against the molar ratio of LPS to Factor C were different from those in the published data. The plots in the dimeric activation model lie on a bell-shaped curve, whatever the Factor C concentration, matching the published data and indicating the appropriateness of that model. We suggest that Factor C is activated by multiple molecular interactions of Factor C with LPS aggregates on which it dimerises and that this explains why larger aggregates are less effective at activating Factor C than smaller ones.
ISSN:0175-7571
1432-1017
DOI:10.1007/s00249-019-01400-4