Imaging Cell‐Matrix Adhesions and Collective Migration of Living Cells by Electrochemiluminescence Microscopy

Cell‐matrix adhesions play essential roles in a variety of biological processes. Herein, we report a label‐free method to map cell‐matrix adhesions of single living cells on an electrode surface by electrochemiluminescence (ECL). An indium tin oxide electrode modified with a silica nanochannel membr...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2020-01, Vol.59 (1), p.449-456
Main Authors: Ding, Hao, Guo, Weiliang, Su, Bin
Format: Article
Language:English
Subjects:
Adhesion
Adhesive strength
Biological activity
cell adhesion
Cell migration
Cell Movement
Cell-Matrix Junctions - chemistry
Cells (biology)
Cells - chemistry
Domains
Electrochemiluminescence
Electrodes
Humans
Indium tin oxides
Luminescent Measurements - methods
microscopy
Microscopy - methods
Nanochannels
Silica
Silicon dioxide
single-cell studies
Spatial distribution
Statistical analysis
Substrates
Tin
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Summary:Cell‐matrix adhesions play essential roles in a variety of biological processes. Herein, we report a label‐free method to map cell‐matrix adhesions of single living cells on an electrode surface by electrochemiluminescence (ECL). An indium tin oxide electrode modified with a silica nanochannel membrane was used as the substrate electrode, at which the ECL generation from freely diffusing luminophores provided a distinct visual contrast between adhesion sites and noncontacted domains, thus selectively revealing the former in a label‐free manner. With this methodology, we studied the spatial distribution, as well as dynamic variation, of cell‐matrix adhesions and the adhesion strength at the subcellular level. Cell‐matrix adhesions of an advancing cell sheet were finally imaged to study the movement of cells in collective migration. A statistical analysis suggests that cells on the far side of leading edge also have the propensity to migrate and do not act as just passive followers. Electrochemiluminescence (ECL) with surface sensitivity was demonstrated to reveal cell‐matrix adhesions of single cells cultured on nanoporous electrode surfaces, as well as their dynamic variation and strength. This approach was further used for imaging cell‐matrix adhesions of an advancing cell sheet to study the moving tendency of cells in collective migration.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201911190