Effects of Isatis root polysaccharide on boar sperm quality during liquid storage and in vitro fertilization

• Published in: Animal reproduction science 2019-11, Vol.210, p.106178-106178, Article 106178
• Main Authors: Ren, Zhiqiang, Shaoyong, Weike, Li, Qian, Ma, Lu, Xiao, Junying, Jiao, Junheng, Yang, Gongshe, Pang, Weijun
• Format: Article
• Language: English
• Subjects: Animals; Boar sperm; Cell Membrane - drug effects; Fertilization in Vitro - veterinary; Isatis - chemistry; Isatis root polysaccharide; IVF; Liquid storage; Male; Membrane Potential, Mitochondrial - drug effects; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Roots - chemistry; Polysaccharides - chemistry; Polysaccharides - pharmacology; Semen Preservation - veterinary; Sperm Motility - drug effects; Spermatozoa - drug effects; Swine
• ISSN: 0378-4320; 1873-2232
• DOI: 10.1016/j.anireprosci.2019.106178

Liquid preservation of boar semen is a preferred method in pig husbandry, and antioxidants to protect against sperm oxidative stress during periods of storage have become the focus of recent research. Through its antioxidant activity, Isatis root polysaccharide (IRPS), a plant extract, can effectively reduce the cellular lipid peroxidation caused by the accumulation of reactive oxygen species inside mitochondria. In the present study, there was examination of the effects of no supplementation (Control) of a semen extender with or supplementation in different concentrations of IRPS (0.2, 0.4, 0.6, 0.8, and 1.2 mg/mL) on sperm quality variables and antioxidant capacity during liquid storage. The results indicate that after prolonged storage (≥ 3 days), the sperm motility was greater in the group supplemented with 0.6 mg/mL IRPS than in the other groups (P