Nocardioides jejuensis sp. nov., isolated from soil

• Published in: Antonie van Leeuwenhoek 2020-03, Vol.113 (3), p.339-347
• Main Authors: Jang, Jun Hwee, Maeng, Soohyun, Jung, Hee-Young, Kim, Myung Kyum, Subramani, Gayathri
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Cardiolipin; Fatty acids; Gene sequencing; Genes; Genomes; Lecithin; Life Sciences; Lipids; Medical Microbiology; Microbiology; New species; Original Paper; Phosphatidylcholine; Phosphatidylethanolamine; Phosphatidylglycerol; Phylogenetics; Phylogeny; Plant Sciences; Pseudogenes; Quinones; rRNA 16S; Soil Science & Conservation; Soils
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-019-01343-y

A Gram-stain positive, asporogenous, aerobic, white -coloured bacterium, designated 18JY15-6 T , was isolated from soil from Jeju Island, Korea. Pairwise analysis of the 16S rRNA gene sequence of strain 18JY15-6 T indicated high similarity to Nocardioides phosphati DSM 104026 T (97.4%), Marmoricola terrae KACC 17308 T (96.7%) and Nocardioides jensenii KCTC 0074BP T (96.6%). Phylogenetic analysis revealed that strain 18JY15-6 T formed a distinct lineage within the family Nocardioidaceae and is closely related to members of the genus Nocardioides . Genome sequencing of strain 18JY15-6 T revealed 3221 total genes, including 3162 protein coding genes, 59 RNA and 31 pseudogenes. Growth was observed at 18–37 °C (optimal 30 °C) in R2A medium at pH 7.0. The major cellular fatty acids of strain 18JY15-6 T were identified as C 16:0 , C 18:1 ω 9 c , C 18:0 10-methyl, tuberculostearic and C 17:0 . The fatty acid profile of strain 18JY15-6 T was more dissimilar when compared with M. terrae. The only respiratory quinone present was found to be MK-8(H 4 ). The major polar lipids are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The results of phylogenetic, biochemical and chemotaxonomic characterisation allow the differentiation of strain 18JY15-6 T from N. phosphati WYH11-7 T , M. terrae JOS5-1 T and N. jensenii NBRC 14755 T which supports the conclusion that this strain represents a novel species of the genus Nocardioides , for which we propose the name Nocardioides jejuensis sp. nov. The type strain of Nocardioides jejuensis is 18JY15-6 T (= KCTC 49105 T  = JCM 33182 T ).